TY - JOUR
T1 - Aberrant splicing of a mouse disabled homolog, mdab1, in the scrambler mouse
AU - Ware, Marcus L.
AU - Fox, Jeremy W.
AU - González, Jorge L.
AU - Davis, Nicole M.
AU - Lambert De Rouvroit, Catherine
AU - Russo, Christopher J.
AU - Chua, Streamson C.
AU - Goffinet, André M.
AU - Walsh, Christopher A.
N1 - Funding Information:
We thank L. Zheng for extensive DNA sequencing assistance; B. Ji, A. Raina, and K. Tan for technical assistance; P. Schwartz for the picture of normal cerebellum; E. Lamperti for help with Northern blotting; K. M. Allen for help with mapping; S. Miles for timely oligonucleotide synthesis; and R. A. Segal, F. Watson, and members of the Walsh laboratory for helpful discussions. M. L. W. was supported by a predoctoral fellowship from the NIGMS. J. L. G. was supported by a fellowship from the Ford Foundation. This work was supported by grants from the NINDS (KO8-NS01520 and RO1-NS32457) to C. A. W., and by the Human Frontier Science Program. C. A. W. is a scholar of the Rita Allen Foundation. C. L. and A. M. G. were supported by grants FRSM 3.4533.95 and ARC 94/99–186.
PY - 1997/7
Y1 - 1997/7
N2 - Although accurate long-distance neuronal migration is a cardinal feature of cerebral cortical development, little is known about control of this migration. The scrambler (scm) mouse shows abnormal cortical lamination that is indistinguishable from reeler. Genetic and physical mapping of scm identified yeast artificial chromosomes containing an exon of mdab1, a homolog of Drosophila disabled, which encodes a phospho-protein that binds nonreceptor tyrosine kinases. mdab1 transcripts showed abnormal splicing in sero homozygotes, with 1.5 kb of intracisternal A particle retrotransposon sequence inserted into the mdab1 coding region in antisense orientation, producing a mutated and truncated predicted protein. Therefore, mdab1 is most likely the scm gene, thus implicating nonreceptor tyrosine kinases in neuronal migration and lamination in developing cerebral cortex.
AB - Although accurate long-distance neuronal migration is a cardinal feature of cerebral cortical development, little is known about control of this migration. The scrambler (scm) mouse shows abnormal cortical lamination that is indistinguishable from reeler. Genetic and physical mapping of scm identified yeast artificial chromosomes containing an exon of mdab1, a homolog of Drosophila disabled, which encodes a phospho-protein that binds nonreceptor tyrosine kinases. mdab1 transcripts showed abnormal splicing in sero homozygotes, with 1.5 kb of intracisternal A particle retrotransposon sequence inserted into the mdab1 coding region in antisense orientation, producing a mutated and truncated predicted protein. Therefore, mdab1 is most likely the scm gene, thus implicating nonreceptor tyrosine kinases in neuronal migration and lamination in developing cerebral cortex.
UR - http://www.scopus.com/inward/record.url?scp=0030868450&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030868450&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)80936-8
DO - 10.1016/S0896-6273(00)80936-8
M3 - Article
C2 - 9292716
AN - SCOPUS:0030868450
SN - 0896-6273
VL - 19
SP - 239
EP - 249
JO - Neuron
JF - Neuron
IS - 2
ER -