A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

Malcolm L. Gefter; David H. Margulies; Matthew D. Scharff

doi:10.1007/BF01551818

A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

Malcolm L. Gefter, David H. Margulies, Matthew D. Scharff

Cell Biology

Research output: Contribution to journal › Article › peer-review

455 Scopus citations

Abstract

A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

Original language	English (US)
Pages (from-to)	231-236
Number of pages	6
Journal	Somatic Cell Genetics
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/BF01551818
State	Published - Mar 1 1977

ASJC Scopus subject areas

Genetics

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10.1007/BF01551818

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title = "A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells",

abstract = "A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.",

author = "Gefter, {Malcolm L.} and Margulies, {David H.} and Scharff, {Matthew D.}",

year = "1977",

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T1 - A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

AU - Gefter, Malcolm L.

AU - Margulies, David H.

AU - Scharff, Matthew D.

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Y1 - 1977/3/1

N2 - A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

AB - A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

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A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

Abstract

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