A sandwiched ventricular explant assay to model mouse coronary angiogenesis ex vivo

Pengfei Lu; Bingruo Wu; Yidong Wang; Jingran Zhang; Bin Zhou

doi:10.1016/j.xpro.2023.102619

A sandwiched ventricular explant assay to model mouse coronary angiogenesis ex vivo

Pengfei Lu, Bingruo Wu, Yidong Wang, Jingran Zhang, Bin Zhou

Genetics

Research output: Contribution to journal › Article › peer-review

Abstract

Developing an ex vivo system that mimics in vivo developmental coronary angiogenesis provides an improved understanding of its underlying molecular and cellular mechanisms. Here, we present a sandwiched embryonic ventricular explant assay to model mouse coronary angiogenesis ex vivo. We describe steps for breeding mice, labeling endocardial cells, isolating murine hearts, dissecting left ventricles, and making sandwiched explants in Matrigel. We then detail procedures for modeling coronary angiogenesis and taking images. For complete details on the use and execution of this protocol, please refer to Lu et al.

Original language	English (US)
Article number	102619
Journal	STAR Protocols
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2023.102619
State	Published - Dec 15 2023

Keywords

Cell Culture
Cell Differentiation
Developmental Biology

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2023.102619

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abstract = "Developing an ex vivo system that mimics in vivo developmental coronary angiogenesis provides an improved understanding of its underlying molecular and cellular mechanisms. Here, we present a sandwiched embryonic ventricular explant assay to model mouse coronary angiogenesis ex vivo. We describe steps for breeding mice, labeling endocardial cells, isolating murine hearts, dissecting left ventricles, and making sandwiched explants in Matrigel. We then detail procedures for modeling coronary angiogenesis and taking images. For complete details on the use and execution of this protocol, please refer to Lu et al.",

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AU - Zhang, Jingran

AU - Zhou, Bin

PY - 2023/12/15

Y1 - 2023/12/15

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AB - Developing an ex vivo system that mimics in vivo developmental coronary angiogenesis provides an improved understanding of its underlying molecular and cellular mechanisms. Here, we present a sandwiched embryonic ventricular explant assay to model mouse coronary angiogenesis ex vivo. We describe steps for breeding mice, labeling endocardial cells, isolating murine hearts, dissecting left ventricles, and making sandwiched explants in Matrigel. We then detail procedures for modeling coronary angiogenesis and taking images. For complete details on the use and execution of this protocol, please refer to Lu et al.

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KW - Cell Differentiation

KW - Developmental Biology

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A sandwiched ventricular explant assay to model mouse coronary angiogenesis ex vivo

Abstract

Keywords

ASJC Scopus subject areas

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