@article{43f11871692c46139bc8ad1be0462253,
title = "A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition",
abstract = "There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition.",
keywords = "ACE2, COVID-19, SARS-CoV-2, VSV, antiviral drugs, convalescent plasma, neutralization assay, neutralizing antibody, serology, surrogate",
author = "Dieterle, {M. Eugenia} and Denise Haslwanter and Bortz, {Robert H.} and Wirchnianski, {Ariel S.} and Gorka Lasso and Olivia Vergnolle and Abbasi, {Shawn A.} and Fels, {J. Maximilian} and Ethan Laudermilch and Catalina Florez and Amanda Mengotto and Duncan Kimmel and Malonis, {Ryan J.} and George Georgiev and Jose Quiroz and Jason Barnhill and Pirofski, {Liise anne} and Daily, {Johanna P.} and Dye, {John M.} and Lai, {Jonathan R.} and Herbert, {Andrew S.} and Kartik Chandran and Jangra, {Rohit K.}",
note = "Funding Information: We thank I. Gutierrez, E. Valencia, L. Polanco, and S. Diaz for laboratory management and technical support. We thank J. McLellan for his generous gifts of wild-type and recombinant SARS-CoV-2 spike constructs. We also thank F. Krammer, H. Choe, and R. Reeves for their generous gifts of SARS-CoV-2 RBD, hACE2, and TMPRSS2 constructs, respectively. We are grateful to J. Carette for his generous gift of Huh-7.5.1 cells and to S. Anthony, E. Choi, B. Gomperts, B. Maniccasamy, K. Stapleford, and C. Sen for their generous gifts of airway epithelial cell lines. This work was supported in part by National Institutes of Health (NIH) grants U19AI142777 and R01AI132633 (to K.C.), R01AI143453 and R01AI123654 (to L.P.), R01AI125462 (to J.R.L.) and R21AI141367 (to J.P.D). M.E.D. is a Latin American Fellow in the Biomedical Sciences, supported by the Pew Charitable Trusts . R.H.B. and R.J.M. were partially supported by NIH training grant 2T32GM007288-45 (Medical Scientist Training Program) at Albert Einstein College of Medicine. Opinions, conclusions, interpretations, and recommendations are those of the authors and are not necessarily endorsed by the U.S. Army. The mention of trade names or commercial products does not constitute endorsement or recommendation for use by the Department of the Army or the Department of Defense. Funding Information: We thank I. Gutierrez, E. Valencia, L. Polanco, and S. Diaz for laboratory management and technical support. We thank J. McLellan for his generous gifts of wild-type and recombinant SARS-CoV-2 spike constructs. We also thank F. Krammer, H. Choe, and R. Reeves for their generous gifts of SARS-CoV-2 RBD, hACE2, and TMPRSS2 constructs, respectively. We are grateful to J. Carette for his generous gift of Huh-7.5.1 cells and to S. Anthony, E. Choi, B. Gomperts, B. Maniccasamy, K. Stapleford, and C. Sen for their generous gifts of airway epithelial cell lines. This work was supported in part by National Institutes of Health (NIH) grants U19AI142777 and R01AI132633 (to K.C.), R01AI143453 and R01AI123654 (to L.P.), R01AI125462 (to J.R.L.) and R21AI141367 (to J.P.D). M.E.D. is a Latin American Fellow in the Biomedical Sciences, supported by the Pew Charitable Trusts. R.H.B. and R.J.M. were partially supported by NIH training grant 2T32GM007288-45 (Medical Scientist Training Program) at Albert Einstein College of Medicine. Opinions, conclusions, interpretations, and recommendations are those of the authors and are not necessarily endorsed by the U.S. Army. The mention of trade names or commercial products does not constitute endorsement or recommendation for use by the Department of the Army or the Department of Defense. Conceptualization, M.E.D. D.H. K.C. R.K.J.; Methodology, M.E.D. D.H. J.M.D. A.S.H. K.C. R.K.J.; Investigation, M.E.D. D.H. R.H.B. G.L. O.V. S.A.A. A.S.H. R.K.J.; Resources, M.E.D. D.H. R.H.B. A.S.W. O.V. J.M.F. E.L. C.F. R.J.M. G.G. L.-a.P. J.P.D. J.M.D. J.R.L. K.C. R.K.J.; Data Curation, M.E.D. D.H. R.H.B. A.S.W. E.L. C.F. A.M. D.K. J.Q. K.C. R.K.J.; Writing?Original Draft, M.E.D. D.H. A.S.H. K.C. R.K.J.; Writing?Review & Editing, all authors; Visualization, M.E.D. D.H. R.H.B. G.L. K.C. R.K.J.; Supervision, L.-a.P. J.P.D. J.M.D. J.R.L. A.S.H. K.C. R.K.J.; Project administration, K.C. R.K.J.; Funding Acquisition, J.B. L.-a. P. J.P.D. J.M.D. J.R.L. K.C. K.C. is a member of the scientific advisory board of Integrum Scientific, LLC. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = sep,
day = "9",
doi = "10.1016/j.chom.2020.06.020",
language = "English (US)",
volume = "28",
pages = "486--496.e6",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "3",
}
