The transcription termination site for yeast RNA polymerase I requires not only an 11 bp binding site for Reb1p, but also about 46 bp of 5′ flanking sequence. We propose that Reb1p bound to its site is part of a pause element, while the 5′ flanking sequence contains a release element. Pausing requires little other than the DNA-binding domain of Reb1p and is not specific for polymerase I. The release element, however, can be polymerase specific. We propose a general model for eukaryotic transcription terminators in which termination occurs when a relatively nonspecific signal induces polymerase to pause in the context of a release element.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)