Abstract
Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.
Original language | English (US) |
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Pages (from-to) | 33-37 |
Number of pages | 5 |
Journal | International Journal for Parasitology |
Volume | 42 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2012 |
Keywords
- Chagas disease
- Destabilisation domain
- Genetic manipulation
- PTREX vector
- Protein expression
- Trypanosoma cruzi
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases