@article{4eff900e250349a8946f7aa3ff264cd3,
title = "A high-throughput strategy for dissecting mammalian genetic interactions",
abstract = "Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPRCas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification. This approach is an important step towards elucidating genetic networks relevant to human diseases and the development of more efficient Cas9-based therapeutics.",
author = "Stockman, {Victoria B.} and Lila Ghamsari and Gorka Lasso and Barry Honig and Shapira, {Sagi D.} and Wang, {Harris H.}",
note = "Funding Information: H.H.W. acknowledges funding from NIH (1DP5OD009172-02, 1U01GM110714-01A1), NSF (MCB-1453219), Sloan Foundation (FR-2015-65795), DARPA (W911NF-15-2-0065), and ONR (N00014-15-1-2704). S.S. acknowledges funding from NIH R01 (GM109018-04) and NIH U54 (CA121852-07). We thank Beatrice Leitao, Nathan Johns and Jacky Cheung for technical assistance and members of the Shapira and Wang labs for helpful discussions. We additionally thank Michael Metzger and Stephen Goff for providing reagents including an eGFP cell line. Publisher Copyright: {\textcopyright} 2016 Stockman et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2016",
month = dec,
doi = "10.1371/journal.pone.0167617",
language = "English (US)",
volume = "11",
journal = "PloS one",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",
}
