A high-resolution radiation hybrid map of the human genome draft sequence

M. Olivier; A. Aggarwal; J. Allen; A. A. Almendras; E. S. Bajorek; E. M. Beasley; S. D. Brady; J. M. Bushard; V. I. Bustos; A. Chu; T. R. Chung; A. De Witte; M. E. Denys; R. Dominguez; N. Y. Fang; B. D. Foster; R. W. Freudenberg; D. Hadley; L. R. Hamilton; T. J. Jeffrey; L. Kelly; L. Lazzeroni; M. R. Levy; S. C. Lewis; X. Liu; F. J. Lopez; B. Louie; J. P. Marquis; R. A. Martinez; M. K. Matsuura; N. S. Misherghi; J. A. Norton; A. Olshen; S. M. Perkins; A. J. Perou; C. Piercy; M. Piercy; F. Qin; T. Reif; K. Sheppard; V. Shokoohi; G. A. Smick; W. L. Sun; E. A. Stewart; J. Fernando Tejeda; N. M. Tran; T. Trejo; N. T. Vo; S. C.M. Yan; D. L. Zierten; S. Zhao; R. Sachidanandam; B. J. Trask; R. M. Myers; D. R. Cox

doi:10.1126/science.1057437

A high-resolution radiation hybrid map of the human genome draft sequence

M. Olivier, A. Aggarwal, J. Allen, A. A. Almendras, E. S. Bajorek, E. M. Beasley, S. D. Brady, J. M. Bushard, V. I. Bustos, A. Chu, T. R. Chung, A. De Witte, M. E. Denys, R. Dominguez, N. Y. Fang, B. D. Foster, R. W. Freudenberg, D. Hadley, L. R. Hamilton, T. J. JeffreyL. Kelly, L. Lazzeroni, M. R. Levy, S. C. Lewis, X. Liu, F. J. Lopez, B. Louie, J. P. Marquis, R. A. Martinez, M. K. Matsuura, N. S. Misherghi, J. A. Norton, A. Olshen, S. M. Perkins, A. J. Perou, C. Piercy, M. Piercy, F. Qin, T. Reif, K. Sheppard, V. Shokoohi, G. A. Smick, W. L. Sun, E. A. Stewart, J. Fernando Tejeda, N. M. Tran, T. Trejo, N. T. Vo, S. C.M. Yan, D. L. Zierten, S. Zhao, R. Sachidanandam, B. J. Trask, R. M. Myers, D. R. Cox

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Abstract

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

Original language	English (US)
Pages (from-to)	1298-1302
Number of pages	5
Journal	Science
Volume	291
Issue number	5507
DOIs	https://doi.org/10.1126/science.1057437
State	Published - Feb 16 2001
Externally published	Yes

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Olivier, M., Aggarwal, A., Allen, J., Almendras, A. A., Bajorek, E. S., Beasley, E. M., Brady, S. D., Bushard, J. M., Bustos, V. I., Chu, A., Chung, T. R., De Witte, A., Denys, M. E., Dominguez, R., Fang, N. Y., Foster, B. D., Freudenberg, R. W., Hadley, D., Hamilton, L. R., ... Cox, D. R. (2001). A high-resolution radiation hybrid map of the human genome draft sequence. Science, 291(5507), 1298-1302. https://doi.org/10.1126/science.1057437

Olivier, M, Aggarwal, A, Allen, J, Almendras, AA, Bajorek, ES, Beasley, EM, Brady, SD, Bushard, JM, Bustos, VI, Chu, A, Chung, TR, De Witte, A, Denys, ME, Dominguez, R, Fang, NY, Foster, BD, Freudenberg, RW, Hadley, D, Hamilton, LR, Jeffrey, TJ, Kelly, L, Lazzeroni, L, Levy, MR, Lewis, SC, Liu, X, Lopez, FJ, Louie, B, Marquis, JP, Martinez, RA, Matsuura, MK, Misherghi, NS, Norton, JA, Olshen, A, Perkins, SM, Perou, AJ, Piercy, C, Piercy, M, Qin, F, Reif, T, Sheppard, K, Shokoohi, V, Smick, GA, Sun, WL, Stewart, EA, Fernando Tejeda, J, Tran, NM, Trejo, T, Vo, NT, Yan, SCM, Zierten, DL, Zhao, S, Sachidanandam, R, Trask, BJ, Myers, RM & Cox, DR 2001, 'A high-resolution radiation hybrid map of the human genome draft sequence', Science, vol. 291, no. 5507, pp. 1298-1302. https://doi.org/10.1126/science.1057437

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abstract = "We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.",

author = "M. Olivier and A. Aggarwal and J. Allen and Almendras, {A. A.} and Bajorek, {E. S.} and Beasley, {E. M.} and Brady, {S. D.} and Bushard, {J. M.} and Bustos, {V. I.} and A. Chu and Chung, {T. R.} and {De Witte}, A. and Denys, {M. E.} and R. Dominguez and Fang, {N. Y.} and Foster, {B. D.} and Freudenberg, {R. W.} and D. Hadley and Hamilton, {L. R.} and Jeffrey, {T. J.} and L. Kelly and L. Lazzeroni and Levy, {M. R.} and Lewis, {S. C.} and X. Liu and Lopez, {F. J.} and B. Louie and Marquis, {J. P.} and Martinez, {R. A.} and Matsuura, {M. K.} and Misherghi, {N. S.} and Norton, {J. A.} and A. Olshen and Perkins, {S. M.} and Perou, {A. J.} and C. Piercy and M. Piercy and F. Qin and T. Reif and K. Sheppard and V. Shokoohi and Smick, {G. A.} and Sun, {W. L.} and Stewart, {E. A.} and {Fernando Tejeda}, J. and Tran, {N. M.} and T. Trejo and Vo, {N. T.} and Yan, {S. C.M.} and Zierten, {D. L.} and S. Zhao and R. Sachidanandam and Trask, {B. J.} and Myers, {R. M.} and Cox, {D. R.}",

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T1 - A high-resolution radiation hybrid map of the human genome draft sequence

AU - Olivier, M.

AU - Aggarwal, A.

AU - Allen, J.

AU - Almendras, A. A.

AU - Bajorek, E. S.

AU - Beasley, E. M.

AU - Brady, S. D.

AU - Bushard, J. M.

AU - Bustos, V. I.

AU - Chu, A.

AU - Chung, T. R.

AU - De Witte, A.

AU - Denys, M. E.

AU - Dominguez, R.

AU - Fang, N. Y.

AU - Foster, B. D.

AU - Freudenberg, R. W.

AU - Hadley, D.

AU - Hamilton, L. R.

AU - Jeffrey, T. J.

AU - Kelly, L.

AU - Lazzeroni, L.

AU - Levy, M. R.

AU - Lewis, S. C.

AU - Liu, X.

AU - Lopez, F. J.

AU - Louie, B.

AU - Marquis, J. P.

AU - Martinez, R. A.

AU - Matsuura, M. K.

AU - Misherghi, N. S.

AU - Norton, J. A.

AU - Olshen, A.

AU - Perkins, S. M.

AU - Perou, A. J.

AU - Piercy, C.

AU - Piercy, M.

AU - Qin, F.

AU - Reif, T.

AU - Sheppard, K.

AU - Shokoohi, V.

AU - Smick, G. A.

AU - Sun, W. L.

AU - Stewart, E. A.

AU - Fernando Tejeda, J.

AU - Tran, N. M.

AU - Trejo, T.

AU - Vo, N. T.

AU - Yan, S. C.M.

AU - Zierten, D. L.

AU - Zhao, S.

AU - Sachidanandam, R.

AU - Trask, B. J.

AU - Myers, R. M.

AU - Cox, D. R.

PY - 2001/2/16

Y1 - 2001/2/16

N2 - We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

AB - We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

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DO - 10.1126/science.1057437

M3 - Article

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SN - 0036-8075

VL - 291

SP - 1298

EP - 1302

JO - Science

JF - Science

IS - 5507

ER -

A high-resolution radiation hybrid map of the human genome draft sequence

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