Engineering Sortase Variants for Intracellular and Cell Surface Labeling

  • Levy, Matthew (PI)

Project: Research project

Project Details

Description

DESCRIPTION (provided by applicant): We propose to develop a new system for site specific labeling proteins in live cells based on the bacterial transpeptidase sortase A (SrtA). Using a combination of protein engineering and directed evolution, we will develop SrtA variants capable of efficiently functioning within mammalian cells. We will also develop exceptionally small (
StatusFinished
Effective start/end date9/30/098/31/15

Funding

  • National Institute of General Medical Sciences: $309,124.00
  • National Institute of General Medical Sciences: $298,304.00
  • National Institute of General Medical Sciences: $312,246.00
  • National Institute of General Medical Sciences: $309,124.00
  • National Institute of General Medical Sciences: $290,320.00

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.