Project Details
Description
This proposal focuses on the development of non-radioisotopic in situ
hybridization assay to detect LAV/HTLV-III infected cells, and to develop a
method for recovery of AIDS virus from infected sources using a T4+ cell
line that expresses the transactivating translational gene (tat) of
LAV/HTLV-III as the basis of a virus detection/culture system. The offeror
emphasize that in situ hybridization offers advantages over immunologic
methods of detecting virus infected cells since it is able to detect
infected cells which are not expressing viral proteins. Their method for
in situ hybridization uses non-isotopic detection which allows easy
visualization of infected cells by standard microscopy within one working
day. The objectives of the current proposal are to simplify the method so
that it can be adapted for clinical use, and to improve the detection to
the point where a single copy of virus DNA or RNA can be detected.
Status | Finished |
---|---|
Effective start/end date | 12/31/89 → 9/30/95 |
ASJC
- Medicine(all)
- Infectious Diseases
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