TY - JOUR
T1 - Zinc homeostasis may reverse the synergistic neurotoxicity of heavy metal mixtures in Caenorhabditis elegans
AU - Cai, Haiqing
AU - Bao, Yu
AU - Cheng, Hong
AU - Ge, Xiaoting
AU - Zhang, Mengdi
AU - Feng, Xiuming
AU - Zheng, Yuan
AU - He, Junxiu
AU - Wei, Yue
AU - Liu, Chaoqun
AU - Li, Longman
AU - Huang, Lulu
AU - Wang, Fei
AU - Chen, Xing
AU - Chen, Pan
AU - Yang, Xiaobo
N1 - Publisher Copyright:
© 2023
PY - 2023/4/10
Y1 - 2023/4/10
N2 - Heavy metal mixtures can cause nerve damage. However, the combined effects of metal mixtures are extremely complex and rarely studied. Zinc (Zn) homeostasis plays an integral role in neural function, but the role of Zn homeostasis in the toxicity of metal mixtures is not well understood. Here, we investigated the combined effects of manganese (Mn), lead (Pb) and arsenic (As) on nerves and the effect of Zn homeostasis on metal toxicity. Caenorhabditis elegans (Maupas, 1900) were exposed to single and multiple metals for 8 days, their movement, behavior, neurons and metal concentration were detected to evaluate the combined effect of metal mixtures. After nematodes were co-treated with metal mixtures and Zn, the nerve function, Zn concentration and redox balance were detected to evaluate the effect of Zn homeostasis on metal toxicity. The results showed that Mn + Pb and Pb + As mixtures induced synergistic toxicity for nematode nerves, which damaged movement, behavior and neurons, and decreased Zn concentration. While Zn supplementation recovered Zn homeostasis and promoted redox balance on nematodes, and then improved the nerve function. Our study demonstrated the combined effects of metal mixtures and the neuroprotective effect of Zn homeostasis. Therefore, assessment of metal mixtures toxicity should consider their interaction and the impacts of essential metals homeostasis.
AB - Heavy metal mixtures can cause nerve damage. However, the combined effects of metal mixtures are extremely complex and rarely studied. Zinc (Zn) homeostasis plays an integral role in neural function, but the role of Zn homeostasis in the toxicity of metal mixtures is not well understood. Here, we investigated the combined effects of manganese (Mn), lead (Pb) and arsenic (As) on nerves and the effect of Zn homeostasis on metal toxicity. Caenorhabditis elegans (Maupas, 1900) were exposed to single and multiple metals for 8 days, their movement, behavior, neurons and metal concentration were detected to evaluate the combined effect of metal mixtures. After nematodes were co-treated with metal mixtures and Zn, the nerve function, Zn concentration and redox balance were detected to evaluate the effect of Zn homeostasis on metal toxicity. The results showed that Mn + Pb and Pb + As mixtures induced synergistic toxicity for nematode nerves, which damaged movement, behavior and neurons, and decreased Zn concentration. While Zn supplementation recovered Zn homeostasis and promoted redox balance on nematodes, and then improved the nerve function. Our study demonstrated the combined effects of metal mixtures and the neuroprotective effect of Zn homeostasis. Therefore, assessment of metal mixtures toxicity should consider their interaction and the impacts of essential metals homeostasis.
KW - Caenorhabditis elegans
KW - Metal mixed exposure
KW - Nerve toxicity
KW - Oxidative stress
KW - Zn homeostasis
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UR - http://www.scopus.com/inward/citedby.url?scp=85146667732&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2023.161699
DO - 10.1016/j.scitotenv.2023.161699
M3 - Article
C2 - 36682567
AN - SCOPUS:85146667732
SN - 0048-9697
VL - 868
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 161699
ER -