Abstract
Cell competition, the conditional loss of viable genotypes only when surrounded by other cells, is a phenomenon observed in certain genetic mosaic conditions. We conducted a chemical mutagenesis and screen to recover new mutations that affect cell competition between wild-type and RpS3 heterozygous cells. Mutations were identified by whole-genome sequencing, making use of software tools that greatly facilitate the distinction between newly induced mutations and other sources of apparent sequence polymorphism, thereby reducing false-positive and false-negative identification rates. In addition, we utilized iPLEX MassARRAY for genotyping recombinant chromosomes. These approaches permitted the mapping of a new mutation affecting cell competition when only a single allele existed, with a phenotype assessed only in genetic mosaics, without the benefit of complementation with existing mutations, deletions, or duplications. These techniques expand the utility of chemical mutagenesis and whole-genome sequencing for mutant identification. We discuss mutations in the Atm and Xrpl genes identified in this screen.
Original language | English (US) |
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Pages (from-to) | 3207-3217 |
Number of pages | 11 |
Journal | G3: Genes, Genomes, Genetics |
Volume | 6 |
Issue number | 10 |
DOIs | |
State | Published - 2016 |
Keywords
- Cell competition
- Drosophila melanogaster
- Flybook
- Whole-genome sequencing
- Xrp1
- iPLEX MassARRAY
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Genetics(clinical)