Ventral tegmental microinjection of Δ9-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient

Jianping Chen; Ronen Marmur; Addy Pulles; William Paredes; Eliot L. Gardner

doi:10.1016/0006-8993(93)90298-2

Ventral tegmental microinjection of Δ⁹-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient

Jianping Chen, Ronen Marmur, Addy Pulles, William Paredes, Eliot L. Gardner

Medicine

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ⁹-tetrahydrocannabinol (Δ⁹-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ⁹-THC perfusion (5×10^-5 and 2×10^-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ⁹-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ⁹-THC may result from local actions of Δ⁹-THC at or near the DA terminal projections in Acb.

Original language	English (US)
Pages (from-to)	65-70
Number of pages	6
Journal	Brain research
Volume	621
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(93)90298-2
State	Published - Sep 3 1993

Keywords

Addiction
Brain reward
Cannabinoid
Cannabis
Dopamine
Marijuana
Microdialysis
Nucleus accumbens
Ventral tegmental area
Δ-Tetrahydrocannabinol (Δ-THC)

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(93)90298-2

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Chen, J., Marmur, R., Pulles, A., Paredes, W., & Gardner, E. L. (1993). Ventral tegmental microinjection of Δ⁹-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient. Brain research, 621(1), 65-70. https://doi.org/10.1016/0006-8993(93)90298-2

Ventral tegmental microinjection of Δ⁹-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient. / Chen, Jianping; Marmur, Ronen; Pulles, Addy et al.
In: Brain research, Vol. 621, No. 1, 03.09.1993, p. 65-70.

Research output: Contribution to journal › Article › peer-review

Chen, J, Marmur, R, Pulles, A, Paredes, W & Gardner, EL 1993, 'Ventral tegmental microinjection of Δ⁹-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient', Brain research, vol. 621, no. 1, pp. 65-70. https://doi.org/10.1016/0006-8993(93)90298-2

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title = "Ventral tegmental microinjection of Δ9-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient",

abstract = "Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ9-THC perfusion (5×10-5 and 2×10-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ9-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ9-THC may result from local actions of Δ9-THC at or near the DA terminal projections in Acb.",

keywords = "Addiction, Brain reward, Cannabinoid, Cannabis, Dopamine, Marijuana, Microdialysis, Nucleus accumbens, Ventral tegmental area, Δ-Tetrahydrocannabinol (Δ-THC)",

author = "Jianping Chen and Ronen Marmur and Addy Pulles and William Paredes and Gardner, {Eliot L.}",

note = "Funding Information: Acknowledgements. This study was supported by NIDA Grant DA03622 and NIH Grant RR05397 from the US Public Health Service, NSF Grant BNS-86-09351 from the US National Science Foundation, the Aaron Diamond Foundation, and the New York State Office of Alcoholism and Substance Abuse Services. R.M. is in the Medical Scientist Training Program, Albert Einstein College of Medicine. A.P. is an exchange student from the Faculty of Medicine, University of Utrecht, The Netherlands. J.C. was partially supported by funds from the Sue Golding Graduate Division of Medical Sciences, Albert Einstein College of Medicine. The data in this paper are from a thesis submitted by J.C. in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Neuroscience from the Sue Golding Graduate Division of Medical Sciences, Albert Einstein College of Medicine, Yeshiva University.",

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AB - Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ9-THC perfusion (5×10-5 and 2×10-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ9-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ9-THC may result from local actions of Δ9-THC at or near the DA terminal projections in Acb.

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