TY - JOUR
T1 - Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells
AU - Zeheb, Ron
AU - Beittenmiller, Howard F.
AU - Horwitz, Susan Band
PY - 1987/3/13
Y1 - 1987/3/13
N2 - Three drug-resistant sublines of the murine macrophage-like cell line J774.2 were selected in vitro for their ability to grow in high concentrations of either taxol, vinblastine, or colchicine. Each contains a major plasma membrane glycoprotein (130-150 kDa), which is barely seen in the drug-sensitive parental cell line. Polyclonal antibodies, raised against the glycoproteins present in the colchicine- and vinblastine-resistant cells, were used to probe for relationships among the three glycoproteins. Our observations suggest that the glycoproteins from the different drug-resistant cell lines share many common domains but are not identical.
AB - Three drug-resistant sublines of the murine macrophage-like cell line J774.2 were selected in vitro for their ability to grow in high concentrations of either taxol, vinblastine, or colchicine. Each contains a major plasma membrane glycoprotein (130-150 kDa), which is barely seen in the drug-sensitive parental cell line. Polyclonal antibodies, raised against the glycoproteins present in the colchicine- and vinblastine-resistant cells, were used to probe for relationships among the three glycoproteins. Our observations suggest that the glycoproteins from the different drug-resistant cell lines share many common domains but are not identical.
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U2 - 10.1016/0006-291X(87)91415-X
DO - 10.1016/0006-291X(87)91415-X
M3 - Article
C2 - 3551955
AN - SCOPUS:0023137064
SN - 0006-291X
VL - 143
SP - 732
EP - 739
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -