Up-regulated lncRNA5322 elevates MAPK1 to enhance proliferation of hair follicle stem cells as a ceRNA of microRNA-19b-3p

Bingjie Cai, Xinxin Wang, Hongtao Liu, Shanshan Ma, Kun Zhang, Yanting Zhang, Qinghua Li, Junmin Wang, Minghao Yao, Fangxia Guan, Guangwen Yin

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Hair follicle stem cells (HFSCs), located in the bulge region of the follicle, maintain hair follicle growth and cycling. Long non-coding RNAs (lncRNAs), non-protein coding transcripts, are widely known to play critical roles in differentiation and proliferation of stem cells. Therefore, the current study aimed to explore the regulatory roles of lncRNA5322 in HFSCs proliferation and the underlying regulatory mechanisms. Initially, the expression patterns of lncRNA5322 and microRNA-19b-3p (miR-19b-3p) in HFSCs were detected. Subsequently, gain-and loss-of-functions analyses were conducted to explore the roles of lncRNA5322, miR-19b-3p and mitogen-activated protein kinase 1 (MAPK1) in cell proliferation, colony formation and apoptosis of HFSCs, with the expression of cyclin-dependent kinase (CDK)1 and CDK2 examined. Also, the interaction relationships among lncRNA5322, miR-19b-3p and MAPK1 were explored. Furthermore, a mouse model was established to detect the roles of lncRNA5322, miR-19b-3p, and MAPK1 in wound contraction and epidermal regeneration. Over-expressed lncRNA5322 was found to promote proliferation, colony formation ability but inhibit apoptosis of HFSCs, in addition to up-regulation of the expression of CDK1 and CDK2. LncRNA5322 was found to act as a ceRNA of miR-19b-3p which directly targeted MAPK1. Furthermore, up-regulation of lncRNA5322 enhanced wound contraction and epidermal regeneration in vivo by increasing the expression of MAPK1 through functioning as a ceRNA of miR-19b-3p. In summary, the results in this study suggested that lncRNA5322 serves as a ceRNA of miR-19b-3p to elevate the expression of MAPK1, ultimately promoting HFSCs proliferation, wound contraction and epidermal regeneration of mouse model.

Original languageEnglish (US)
Pages (from-to)1588-1600
Number of pages13
JournalCell Cycle
Volume18
Issue number14
DOIs
StatePublished - Jul 18 2019

Keywords

  • Hair follicle stem cells
  • epidermal regeneration
  • long non-coding RNA5322
  • microRNA-19b-3p
  • mitogen-activated protein kinase 1
  • proliferation
  • wound contraction

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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