Ultrastructural distribution of poly (A)+ RNA during Trypanosoma cruzi-cardiomyocyte interaction in vitro: A quantitative analysis of the total mRNA content by in situ hybridization

Mirian Claudia S. Pereira; Robert H. Singer; Maria De Nazareth S.L. De Meirelles

doi:10.1111/j.1550-7408.2000.tb00046.x

Ultrastructural distribution of poly (A)⁺ RNA during Trypanosoma cruzi-cardiomyocyte interaction in vitro: A quantitative analysis of the total mRNA content by in situ hybridization

Mirian Claudia S. Pereira, Robert H. Singer, Maria De Nazareth S.L. De Meirelles

Anatomy & Structural Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Ultrastructural in situ hybridization was used to visualize the spatial distribution of poly (A)⁺ RNA and quantitate its relative amount within different cellular compartments of cardiomyocytes after T. cruzi infection. The amount of poly (A)⁺ RNA remained about the same up to 24 h post-infection. In contrast, its content was reduced 65% after 72 h of interaction, showing a marked decrease in the cell cytoplasm. This decline in poly (A)⁺ RNA level in host cell cytoplasm was concomitant with intracellular proliferation of T. cruzi amastigotes. Thus, T. cruzi may affect host cell cytoplasmic mRNA stability, associated with the parasite's intracellular multiplication.

Original language	English (US)
Pages (from-to)	264-270
Number of pages	7
Journal	Journal of Eukaryotic Microbiology
Volume	47
Issue number	3
DOIs	https://doi.org/10.1111/j.1550-7408.2000.tb00046.x
State	Published - Jan 1 2000

Keywords

Chagas' disease
Heart muscle cells
Hemoflagellate
Ultrastructural in situ hybridization

ASJC Scopus subject areas

Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1550-7408.2000.tb00046.x

Cite this

Ultrastructural distribution of poly (A)⁺ RNA during Trypanosoma cruzi-cardiomyocyte interaction in vitro: A quantitative analysis of the total mRNA content by in situ hybridization. / Pereira, Mirian Claudia S.; Singer, Robert H.; De Meirelles, Maria De Nazareth S.L.
In: Journal of Eukaryotic Microbiology, Vol. 47, No. 3, 01.01.2000, p. 264-270.

Research output: Contribution to journal › Article › peer-review

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abstract = "Ultrastructural in situ hybridization was used to visualize the spatial distribution of poly (A)+ RNA and quantitate its relative amount within different cellular compartments of cardiomyocytes after T. cruzi infection. The amount of poly (A)+ RNA remained about the same up to 24 h post-infection. In contrast, its content was reduced 65% after 72 h of interaction, showing a marked decrease in the cell cytoplasm. This decline in poly (A)+ RNA level in host cell cytoplasm was concomitant with intracellular proliferation of T. cruzi amastigotes. Thus, T. cruzi may affect host cell cytoplasmic mRNA stability, associated with the parasite's intracellular multiplication.",

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