Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae

E. Anne Martin, Sundas Ijaz, Alberto E. Pereda, Adam C. Miller

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving ethologically relevant behavior in the first five days of development. Here, we describe methods for immunostaining electrical and chemical synapse proteins at 3-7 days post fertilization (dpf) in zebrafish using tricholoracetic acid fixation. The methods presented are ideally suited to easily visualize neural circuits and synapses within the fish.

Original languageEnglish (US)
Article numbere4289
Issue number2
StatePublished - Jan 20 2022


  • Antibody
  • Immunostaining
  • Larvae
  • TCA
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Plant Science


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