Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae

E. Anne Martin; Sundas Ijaz; Alberto E. Pereda; Adam C. Miller

doi:10.21769/BioProtoc.4289

Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae

E. Anne Martin, Sundas Ijaz, Alberto E. Pereda, Adam C. Miller

Neuroscience

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving ethologically relevant behavior in the first five days of development. Here, we describe methods for immunostaining electrical and chemical synapse proteins at 3-7 days post fertilization (dpf) in zebrafish using tricholoracetic acid fixation. The methods presented are ideally suited to easily visualize neural circuits and synapses within the fish.

Original language	English (US)
Article number	e4289
Journal	Bio-protocol
Volume	12
Issue number	2
DOIs	https://doi.org/10.21769/BioProtoc.4289
State	Published - Jan 20 2022

Keywords

Antibody
Immunostaining
Larvae
TCA
Zebrafish

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Neuroscience(all)
Plant Science

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10.21769/BioProtoc.4289

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title = "Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae",

abstract = "Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving ethologically relevant behavior in the first five days of development. Here, we describe methods for immunostaining electrical and chemical synapse proteins at 3-7 days post fertilization (dpf) in zebrafish using tricholoracetic acid fixation. The methods presented are ideally suited to easily visualize neural circuits and synapses within the fish.",

keywords = "Antibody, Immunostaining, Larvae, TCA, Zebrafish",

author = "{Anne Martin}, E. and Sundas Ijaz and Pereda, {Alberto E.} and Miller, {Adam C.}",

note = "Funding Information: This protocol was used in the companion paper Lasseigne et al. (2021) Elife (DOI: 10.7554/eLife.66898). Eunice Kennedy Shriver National Institute of Child Health and Human Development (F32HD102182) to E Anne Martin. National Institute on Deafness and Other Communication Disorders (R01DC011099) to Alberto Pereda. National Institute of Neurological Disorders and Stroke (R21NS085772) to Alberto Pereda. National Institute of Mental Health (RF1MH120016) to Alberto Pereda and Adam C Miller. National Institute of Neurological Disorders and Stroke (R01NS105758) to Adam C Miller. Publisher Copyright: {\textcopyright} Martin et al.",

year = "2022",

month = jan,

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doi = "10.21769/BioProtoc.4289",

language = "English (US)",

volume = "12",

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AU - Anne Martin, E.

AU - Ijaz, Sundas

AU - Pereda, Alberto E.

AU - Miller, Adam C.

N1 - Funding Information: This protocol was used in the companion paper Lasseigne et al. (2021) Elife (DOI: 10.7554/eLife.66898). Eunice Kennedy Shriver National Institute of Child Health and Human Development (F32HD102182) to E Anne Martin. National Institute on Deafness and Other Communication Disorders (R01DC011099) to Alberto Pereda. National Institute of Neurological Disorders and Stroke (R21NS085772) to Alberto Pereda. National Institute of Mental Health (RF1MH120016) to Alberto Pereda and Adam C Miller. National Institute of Neurological Disorders and Stroke (R01NS105758) to Adam C Miller. Publisher Copyright: © Martin et al.

PY - 2022/1/20

Y1 - 2022/1/20

N2 - Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving ethologically relevant behavior in the first five days of development. Here, we describe methods for immunostaining electrical and chemical synapse proteins at 3-7 days post fertilization (dpf) in zebrafish using tricholoracetic acid fixation. The methods presented are ideally suited to easily visualize neural circuits and synapses within the fish.

AB - Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving ethologically relevant behavior in the first five days of development. Here, we describe methods for immunostaining electrical and chemical synapse proteins at 3-7 days post fertilization (dpf) in zebrafish using tricholoracetic acid fixation. The methods presented are ideally suited to easily visualize neural circuits and synapses within the fish.

KW - Antibody

KW - Immunostaining

KW - Larvae

KW - TCA

KW - Zebrafish

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Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae

Abstract

Keywords

ASJC Scopus subject areas

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