TY - JOUR
T1 - Transmission electron microscopy of thin sections of Drosophila
T2 - Preparation of embryos using n-heptane and glutaraldehyde
AU - McDonald, Kent L.
AU - Sharp, David J.
AU - Rickoll, Wayne
PY - 2012/10
Y1 - 2012/10
N2 - This protocol describes the simultaneous permeabilization of Drosophila embryos with n-heptane and initial fixation with glutaraldehyde. Even though the vitelline membrane around the embryo is chemically permeabilized, it must be manually removed to achieve infiltration with embedding resins. Once the embryo is embedded, it can be sectioned for transmission electron microscopy (TEM). This procedure can produce excellent preservation for ultrastructural analysis, and is useful for situations where optimal preservation (e.g., by high-pressure freezing) is not required or is not feasible.
AB - This protocol describes the simultaneous permeabilization of Drosophila embryos with n-heptane and initial fixation with glutaraldehyde. Even though the vitelline membrane around the embryo is chemically permeabilized, it must be manually removed to achieve infiltration with embedding resins. Once the embryo is embedded, it can be sectioned for transmission electron microscopy (TEM). This procedure can produce excellent preservation for ultrastructural analysis, and is useful for situations where optimal preservation (e.g., by high-pressure freezing) is not required or is not feasible.
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U2 - 10.1101/pdb.prot068460
DO - 10.1101/pdb.prot068460
M3 - Article
C2 - 23028066
AN - SCOPUS:84867076900
SN - 1940-3402
VL - 7
SP - 1100
EP - 1103
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 10
ER -