TY - JOUR
T1 - Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT)
AU - Li, Mucong
AU - Beaumont, Nathan
AU - Ma, Chenshuo
AU - Rojas, Juan
AU - Vu, Tri
AU - Harlacher, Max
AU - O'Connell, Graeme
AU - Gessner, Ryan C.
AU - Kilian, Hailey
AU - Kasatkina, Ludmila
AU - Chen, Yong
AU - Huang, Qiang
AU - Shen, Xiling
AU - Lovell, Jonathan F.
AU - Verkhusha, Vladislav V.
AU - Czernuszewicz, Tomek
AU - Yao, Junjie
N1 - Publisher Copyright:
© 1982-2012 IEEE.
PY - 2022/10/1
Y1 - 2022/10/1
N2 - Non-invasive small-animal imaging technologies, such as optical imaging, magnetic resonance imaging and x-ray computed tomography, have enabled researchers to study normal biological phenomena or disease progression in their native conditions. However, existing small-animal imaging technologies often lack either the penetration capability for interrogating deep tissues (e.g., optical microscopy), or the functional and molecular sensitivity for tracking specific activities (e.g., magnetic resonance imaging). To achieve functional and molecular imaging in deep tissues, we have developed an integrated photoacoustic, ultrasound and acoustic angiographic tomography (PAUSAT) system by seamlessly combining light and ultrasound. PAUSAT can perform three imaging modes simultaneously with complementary contrast: high-frequency B-mode ultrasound imaging of tissue morphology, microbubble-enabled acoustic angiography of tissue vasculature, and multi-spectral photoacoustic imaging of molecular probes. PAUSAT can provide three-dimensional (3D) multi-contrast images that are co-registered, with high spatial resolutions at large depths. Using PAUSAT, we performed proof-of-concept in vivo experiments on various small animal models: monitoring longitudinal development of placenta and embryo during mouse pregnancy, tracking biodistribution and metabolism of near-infrared organic dye on the whole-body scale, and detecting breast tumor expressing genetically-encoded photoswitchable phytochromes. These results have collectively demonstrated that PAUSAT has broad applicability in biomedical research, providing comprehensive structural, functional, and molecular imaging of small animal models.
AB - Non-invasive small-animal imaging technologies, such as optical imaging, magnetic resonance imaging and x-ray computed tomography, have enabled researchers to study normal biological phenomena or disease progression in their native conditions. However, existing small-animal imaging technologies often lack either the penetration capability for interrogating deep tissues (e.g., optical microscopy), or the functional and molecular sensitivity for tracking specific activities (e.g., magnetic resonance imaging). To achieve functional and molecular imaging in deep tissues, we have developed an integrated photoacoustic, ultrasound and acoustic angiographic tomography (PAUSAT) system by seamlessly combining light and ultrasound. PAUSAT can perform three imaging modes simultaneously with complementary contrast: high-frequency B-mode ultrasound imaging of tissue morphology, microbubble-enabled acoustic angiography of tissue vasculature, and multi-spectral photoacoustic imaging of molecular probes. PAUSAT can provide three-dimensional (3D) multi-contrast images that are co-registered, with high spatial resolutions at large depths. Using PAUSAT, we performed proof-of-concept in vivo experiments on various small animal models: monitoring longitudinal development of placenta and embryo during mouse pregnancy, tracking biodistribution and metabolism of near-infrared organic dye on the whole-body scale, and detecting breast tumor expressing genetically-encoded photoswitchable phytochromes. These results have collectively demonstrated that PAUSAT has broad applicability in biomedical research, providing comprehensive structural, functional, and molecular imaging of small animal models.
KW - Deep-tissue imaging
KW - acoustic angiography
KW - functional imaging
KW - molecular imaging
KW - photoacoustic imaging
KW - photoswitchable protein
KW - ultrasound imaging
UR - http://www.scopus.com/inward/record.url?scp=85128613037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85128613037&partnerID=8YFLogxK
U2 - 10.1109/TMI.2022.3168859
DO - 10.1109/TMI.2022.3168859
M3 - Article
C2 - 35442884
AN - SCOPUS:85128613037
SN - 0278-0062
VL - 41
SP - 2704
EP - 2714
JO - IEEE Transactions on Medical Imaging
JF - IEEE Transactions on Medical Imaging
IS - 10
ER -