The use of fluorescence quenchers in resonance Raman spectroscopy

Joel M. Friedman; Robin M. Hochstrasser

doi:10.1016/0009-2614(75)80142-4

The use of fluorescence quenchers in resonance Raman spectroscopy

Joel M. Friedman, Robin M. Hochstrasser

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We discuss methods of increasing the resonance Raman signal to noise in the presence of a strong fluorescence background. By considering the time dependence of the competing processes, we conclude that for many commonly encountered systems the Raman signal can be enhanced over the fluorescence by introducing a quencher that decreases the lifetime of the radiative electronic state. The efficacy of this approach is demonstrated by the appearance of resonance Raman from fluorescein in water with the progressive addition of KI.

Original language	English (US)
Pages (from-to)	225-227
Number of pages	3
Journal	Chemical Physics Letters
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/0009-2614(75)80142-4
State	Published - Jun 1 1975
Externally published	Yes

ASJC Scopus subject areas

General Physics and Astronomy
Physical and Theoretical Chemistry

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10.1016/0009-2614(75)80142-4

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title = "The use of fluorescence quenchers in resonance Raman spectroscopy",

abstract = "We discuss methods of increasing the resonance Raman signal to noise in the presence of a strong fluorescence background. By considering the time dependence of the competing processes, we conclude that for many commonly encountered systems the Raman signal can be enhanced over the fluorescence by introducing a quencher that decreases the lifetime of the radiative electronic state. The efficacy of this approach is demonstrated by the appearance of resonance Raman from fluorescein in water with the progressive addition of KI.",

author = "Friedman, {Joel M.} and Hochstrasser, {Robin M.}",

year = "1975",

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AU - Hochstrasser, Robin M.

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Y1 - 1975/6/1

N2 - We discuss methods of increasing the resonance Raman signal to noise in the presence of a strong fluorescence background. By considering the time dependence of the competing processes, we conclude that for many commonly encountered systems the Raman signal can be enhanced over the fluorescence by introducing a quencher that decreases the lifetime of the radiative electronic state. The efficacy of this approach is demonstrated by the appearance of resonance Raman from fluorescein in water with the progressive addition of KI.

AB - We discuss methods of increasing the resonance Raman signal to noise in the presence of a strong fluorescence background. By considering the time dependence of the competing processes, we conclude that for many commonly encountered systems the Raman signal can be enhanced over the fluorescence by introducing a quencher that decreases the lifetime of the radiative electronic state. The efficacy of this approach is demonstrated by the appearance of resonance Raman from fluorescein in water with the progressive addition of KI.

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DO - 10.1016/0009-2614(75)80142-4

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JO - Chemical Physics Letters

JF - Chemical Physics Letters

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ER -

The use of fluorescence quenchers in resonance Raman spectroscopy

Abstract

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