The role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer

Jimmy Belotte; Nicole M. Fletcher; Awoniyi O. Awonuga; Mitchell Alexis; Husam M. Abu-Soud; Mohammed G. Saed; Michael P. Diamond; Ghassan M. Saed

doi:10.1177/1933719113503403

The role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer

Jimmy Belotte
, Nicole M. Fletcher
, Awoniyi O. Awonuga
, Mitchell Alexis
, Husam M. Abu-Soud
, Mohammed G. Saed
, Michael P. Diamond
, Ghassan M. Saed

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Objective: To investigate the role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer (EOC). Methods: Two parent EOC cell lines (MDAH-2774 and SKOV-3) and their chemoresistant counterparts (cisplatin, 50 mmol/L) were used. Total RNA was extracted and subjected to real-time reverse transcriptase polymerase chain reaction to evaluate the expression of glutathione reductase (GSR) and inducible nitric oxide synthase (iNOS), as well as nitrate/nitrite levels. Analysis of variance was used for main effects and Tukey for post hoc analysis at P < .05 for statistical significance. Results: Both cisplatin resistant cell lines displayed a significant decrease in GSR messenger RNA (mRNA) levels and activity (P <.01). As compared to sensitive controls, nitrate/nitrite levels were significantly higher in SKOV-3 cisplatin resistant cells while iNOS mRNA levels were significantly higher in MDAH-2774 cisplatin resistant cells (P < .05). Conclusion: Our data suggest that the development of cisplatin resistance tilts the balance toward a pro-oxidant state in EOC.

Original language	English (US)
Pages (from-to)	503-508
Number of pages	6
Journal	Reproductive Sciences
Volume	21
Issue number	4
DOIs	https://doi.org/10.1177/1933719113503403
State	Published - Apr 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cisplatin resistance
epithelial ovarian cancer
reactive oxygen species

ASJC Scopus subject areas

Obstetrics and Gynecology

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@article{e225a11218874ccbbefab7ab482563f6,

title = "The role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer",

abstract = "Objective: To investigate the role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer (EOC). Methods: Two parent EOC cell lines (MDAH-2774 and SKOV-3) and their chemoresistant counterparts (cisplatin, 50 mmol/L) were used. Total RNA was extracted and subjected to real-time reverse transcriptase polymerase chain reaction to evaluate the expression of glutathione reductase (GSR) and inducible nitric oxide synthase (iNOS), as well as nitrate/nitrite levels. Analysis of variance was used for main effects and Tukey for post hoc analysis at P < .05 for statistical significance. Results: Both cisplatin resistant cell lines displayed a significant decrease in GSR messenger RNA (mRNA) levels and activity (P <.01). As compared to sensitive controls, nitrate/nitrite levels were significantly higher in SKOV-3 cisplatin resistant cells while iNOS mRNA levels were significantly higher in MDAH-2774 cisplatin resistant cells (P < .05). Conclusion: Our data suggest that the development of cisplatin resistance tilts the balance toward a pro-oxidant state in EOC.",

keywords = "cisplatin resistance, epithelial ovarian cancer, reactive oxygen species",

author = "Jimmy Belotte and Fletcher, \{Nicole M.\} and Awonuga, \{Awoniyi O.\} and Mitchell Alexis and Abu-Soud, \{Husam M.\} and Saed, \{Mohammed G.\} and Diamond, \{Michael P.\} and Saed, \{Ghassan M.\}",

note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This project was funded by NIH/NICHD grant K12HD001254 .",

year = "2014",

month = apr,

doi = "10.1177/1933719113503403",

language = "English (US)",

volume = "21",

pages = "503--508",

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T1 - The role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer

AU - Belotte, Jimmy

AU - Fletcher, Nicole M.

AU - Awonuga, Awoniyi O.

AU - Alexis, Mitchell

AU - Abu-Soud, Husam M.

AU - Saed, Mohammed G.

AU - Diamond, Michael P.

AU - Saed, Ghassan M.

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This project was funded by NIH/NICHD grant K12HD001254 .

PY - 2014/4

Y1 - 2014/4

N2 - Objective: To investigate the role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer (EOC). Methods: Two parent EOC cell lines (MDAH-2774 and SKOV-3) and their chemoresistant counterparts (cisplatin, 50 mmol/L) were used. Total RNA was extracted and subjected to real-time reverse transcriptase polymerase chain reaction to evaluate the expression of glutathione reductase (GSR) and inducible nitric oxide synthase (iNOS), as well as nitrate/nitrite levels. Analysis of variance was used for main effects and Tukey for post hoc analysis at P < .05 for statistical significance. Results: Both cisplatin resistant cell lines displayed a significant decrease in GSR messenger RNA (mRNA) levels and activity (P <.01). As compared to sensitive controls, nitrate/nitrite levels were significantly higher in SKOV-3 cisplatin resistant cells while iNOS mRNA levels were significantly higher in MDAH-2774 cisplatin resistant cells (P < .05). Conclusion: Our data suggest that the development of cisplatin resistance tilts the balance toward a pro-oxidant state in EOC.

AB - Objective: To investigate the role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer (EOC). Methods: Two parent EOC cell lines (MDAH-2774 and SKOV-3) and their chemoresistant counterparts (cisplatin, 50 mmol/L) were used. Total RNA was extracted and subjected to real-time reverse transcriptase polymerase chain reaction to evaluate the expression of glutathione reductase (GSR) and inducible nitric oxide synthase (iNOS), as well as nitrate/nitrite levels. Analysis of variance was used for main effects and Tukey for post hoc analysis at P < .05 for statistical significance. Results: Both cisplatin resistant cell lines displayed a significant decrease in GSR messenger RNA (mRNA) levels and activity (P <.01). As compared to sensitive controls, nitrate/nitrite levels were significantly higher in SKOV-3 cisplatin resistant cells while iNOS mRNA levels were significantly higher in MDAH-2774 cisplatin resistant cells (P < .05). Conclusion: Our data suggest that the development of cisplatin resistance tilts the balance toward a pro-oxidant state in EOC.

KW - cisplatin resistance

KW - epithelial ovarian cancer

KW - reactive oxygen species

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The role of oxidative stress in the development of cisplatin resistance in epithelial ovarian cancer

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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