The murine N-ras gene is not essential for growth and development

Heywood Umanoff, Winfried Edelmann, Angel Pellicer, Raju Kucherlapati

Research output: Contribution to journalArticlepeer-review

208 Scopus citations


The mammalian ras gene family encodes key cell-signaling, cell growth- related proteins that have been highly conserved in species from yeast to man. Specific point mutations in the ras genes are associated with various mammalian tumors. To understand the developmental role of the N-ras protooncogene in the mouse, we have disrupted its gene function by homologous recombination in embryonic stem cells. Mice derived from these cells that are homozygous for the N-ras mutation do not produce any detectable N-Ras protein and are morphologically and histologically indistinguishable from their heterozygous and wild-type siblings. Since N-ras is expressed at high levels in hematopoietic cells, we examined different populations of cells in peripheral blood and found no differences between mutant and normal animals. Our results show that N-ras gene function is dispensable for normal mouse development, growth, and fertility.

Original languageEnglish (US)
Pages (from-to)1709-1713
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number5
StatePublished - Feb 28 1995


  • embryonic stem cells
  • gene targeting
  • oncogenes

ASJC Scopus subject areas

  • General


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