TY - JOUR
T1 - The mouse histone H1 genes
T2 - Gene organization and differential regulation
AU - Wang, Zeng Feng
AU - Sirotkin, Allen M.
AU - Buchold, Gregory M.
AU - Skoultchi, Arthur I.
AU - Marzluff, William F.
N1 - Funding Information:
This work was supported by NIH grants GM29832 to W.F.M. and CA16368 to A.I.S. A.M.S. was supported by NIH training grant GM07491. We thank Dr Myra Hurt for providing purified histone H1b protein, Lee Johnson for providing the RNA from 3T6 cells, Yen Li and Yue Xiong for providing the RNA from the C2C12 cells, Hua Wu and Terry Van Dyke for providing mouse tissue, and Edward Nieves and Ruth Angeletti for performing TOF-MS analyses. We thank Dr Leonard Cohen for helpful discussions.
PY - 1997/8/8
Y1 - 1997/8/8
N2 - There are six mouse histone H1 genes present in the histone gene cluster on mouse chromosome 13. These genes encode five histone H1 variants expressed in somatic cells, H1a to H1e, and the testis-specific H1t histone. Two of the genes that have not been assigned previously to the five somatic H1 subtypes have been identified as encoding the H1b and H1d subtypes. Three of the H1 genes, H1a, H1e and H1t, are present on an 80 kb segment of DNA that contains nine core histone genes. Two others, Hid and H1e, are present in a second patch, while the H1b gene is at least 500 kb away in a patch containing 14 core histone genes. The histone H1 genes are differentially expressed. All five genes for the somatic histone H1 proteins are expressed in exponentially growing cells. However, the levels of H1a, H1b and H1d mRNAs are greatly reduced in cells that are terminally differentiated or arrested in G0, while the H1e and H1e mRNAs continue to be expressed. In addition to the major RNA that ends at the stem-loop, the H1c gene expresses a longer, polyadenylated mRNA in differentiated cells, although in varying amounts. None of the other histone H1 genes encodes detectable amounts of polyadenylated mRNAs.
AB - There are six mouse histone H1 genes present in the histone gene cluster on mouse chromosome 13. These genes encode five histone H1 variants expressed in somatic cells, H1a to H1e, and the testis-specific H1t histone. Two of the genes that have not been assigned previously to the five somatic H1 subtypes have been identified as encoding the H1b and H1d subtypes. Three of the H1 genes, H1a, H1e and H1t, are present on an 80 kb segment of DNA that contains nine core histone genes. Two others, Hid and H1e, are present in a second patch, while the H1b gene is at least 500 kb away in a patch containing 14 core histone genes. The histone H1 genes are differentially expressed. All five genes for the somatic histone H1 proteins are expressed in exponentially growing cells. However, the levels of H1a, H1b and H1d mRNAs are greatly reduced in cells that are terminally differentiated or arrested in G0, while the H1e and H1e mRNAs continue to be expressed. In addition to the major RNA that ends at the stem-loop, the H1c gene expresses a longer, polyadenylated mRNA in differentiated cells, although in varying amounts. None of the other histone H1 genes encodes detectable amounts of polyadenylated mRNAs.
KW - Chromatin
KW - Gene mapping
KW - Histone
KW - Histone H1
KW - Histone mRNA
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U2 - 10.1006/jmbi.1997.1166
DO - 10.1006/jmbi.1997.1166
M3 - Article
C2 - 9300059
AN - SCOPUS:0031559570
SN - 0022-2836
VL - 271
SP - 124
EP - 138
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -