TY - JOUR
T1 - The LIM homeobox gene ceh-14 confers thermosensory function to the AFD neurons in Caenorhabditis elegans
AU - Cassata, Giuseppe
AU - Kagoshima, Hiroshi
AU - Andachi, Yoshiki
AU - Kohara, Yuji
AU - Dürrenberger, Markus B.
AU - Hall, David H.
AU - Bürglin, Thomas R.
N1 - Funding Information:
We would like to thank Gisela Niklaus and Ursula Sauder for excellent technical support. We would also like to thank Peter Okkema, Cara Coburn, Cori Bargmann, and Andy Fire for sharing vectors, cDNAs, and libraries, and Cori Bargmann and Oliver Hobert for critical comments. Also, thanks to Franziska Kuhn for help with RNA and protein work and Eileen A. German for electron microscopy measurements. Some nematode strains were provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health (NIH) National Center for Research Resources. This work and H. K. have received support from the Novartis Foundation, from Jeff Schatz, and the Sandoz Stiftung. H. K. is a recipient of a JSPS Postdoctoral Fellowship for Research Abroad. T. R. B. is supported by grant NF. 3130-038786.93. D. H. H. and the Center for C. elegans Anatomy are funded by NIH grant RR12596. This work was supported by grants NF. 3100-040843.94 and NF. 31-50839.97 from the Swiss National Science Foundation and the University of Basel, Kanton Basel-Stadt.
PY - 2000
Y1 - 2000
N2 - In Caenorhabditis elegans three pairs of neurons, AFD, AIY, and AIZ, play a key role in thermosensation. The LIM homeobox gene ceh-14 is expressed in the AFD thermosensory neurons, ceh-14 mutant animals display athermotactic behaviors, although the neurons are still present and differentiated. Two other LIM homeobox genes, ttx-3 and lin-11, function in the two interneurons AIY and AIZ, respectively. Thus, the three key thermosensory neurons are specified by three different LIM homeobox genes, ceh-14 ttx-3 lin-11 triple mutant animals have a basic cryophilic thermotaxis behavior indicative of a second thermotaxis pathway. Misexpression of ceh-14 in chemosensory neurons can restore thermotacfic behavior without impairing the chemosensory function. Thus, ceh-14 confers thermosensory function to neurons.
AB - In Caenorhabditis elegans three pairs of neurons, AFD, AIY, and AIZ, play a key role in thermosensation. The LIM homeobox gene ceh-14 is expressed in the AFD thermosensory neurons, ceh-14 mutant animals display athermotactic behaviors, although the neurons are still present and differentiated. Two other LIM homeobox genes, ttx-3 and lin-11, function in the two interneurons AIY and AIZ, respectively. Thus, the three key thermosensory neurons are specified by three different LIM homeobox genes, ceh-14 ttx-3 lin-11 triple mutant animals have a basic cryophilic thermotaxis behavior indicative of a second thermotaxis pathway. Misexpression of ceh-14 in chemosensory neurons can restore thermotacfic behavior without impairing the chemosensory function. Thus, ceh-14 confers thermosensory function to neurons.
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U2 - 10.1016/S0896-6273(00)81062-4
DO - 10.1016/S0896-6273(00)81062-4
M3 - Article
C2 - 10774727
AN - SCOPUS:0033681487
SN - 0896-6273
VL - 25
SP - 587
EP - 597
JO - Neuron
JF - Neuron
IS - 3
ER -