The effect of adenosine analogues on the in vitro growth of trypanosoma cruzi

Jeffrey P. Nadler; Edgar Lederer; Murray Wittner; Stephen G. Baum; Herbert B. Tanowitz

doi:10.1016/0035-9203(82)90169-9

The effect of adenosine analogues on the in vitro growth of trypanosoma cruzi

Jeffrey P. Nadler
, Edgar Lederer
, Murray Wittner
, Stephen G. Baum
, Herbert B. Tanowitz

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Trypanosoma cruzi (Brazil strain) was maintained in liver infusion tryptose medium supplemented with 5 or 10% foetal calf serum at 27 °C on a rotating shaker platform. 85 to 95% of the organisms under these conditions are epimastigotes and the medium supported logarithmic growth for up to 24 hours. The effect of S-isobutyl adenosine and Sinefungin^R against cultured T. cruzi epimastigotes was studied: growth rate was slowed by both in a dose-dependent fashion; 500 μm Sinefungin caused complete inhibition which was irreversible after 24-hour exposure but the effect of S-isobutyl adenosine (1000 μm) was reversible. Motility and morphology appeared to be unaffected.

Original language	English (US)
Pages (from-to)	285-287
Number of pages	3
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	76
Issue number	3
DOIs	https://doi.org/10.1016/0035-9203(82)90169-9
State	Published - 1982

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0035-9203(82)90169-9

Cite this

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title = "The effect of adenosine analogues on the in vitro growth of trypanosoma cruzi",

abstract = "Trypanosoma cruzi (Brazil strain) was maintained in liver infusion tryptose medium supplemented with 5 or 10\% foetal calf serum at 27 °C on a rotating shaker platform. 85 to 95\% of the organisms under these conditions are epimastigotes and the medium supported logarithmic growth for up to 24 hours. The effect of S-isobutyl adenosine and SinefunginR against cultured T. cruzi epimastigotes was studied: growth rate was slowed by both in a dose-dependent fashion; 500 μm Sinefungin caused complete inhibition which was irreversible after 24-hour exposure but the effect of S-isobutyl adenosine (1000 μm) was reversible. Motility and morphology appeared to be unaffected.",

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T1 - The effect of adenosine analogues on the in vitro growth of trypanosoma cruzi

AU - Nadler, Jeffrey P.

AU - Lederer, Edgar

AU - Wittner, Murray

AU - Baum, Stephen G.

AU - Tanowitz, Herbert B.

PY - 1982

Y1 - 1982

N2 - Trypanosoma cruzi (Brazil strain) was maintained in liver infusion tryptose medium supplemented with 5 or 10% foetal calf serum at 27 °C on a rotating shaker platform. 85 to 95% of the organisms under these conditions are epimastigotes and the medium supported logarithmic growth for up to 24 hours. The effect of S-isobutyl adenosine and SinefunginR against cultured T. cruzi epimastigotes was studied: growth rate was slowed by both in a dose-dependent fashion; 500 μm Sinefungin caused complete inhibition which was irreversible after 24-hour exposure but the effect of S-isobutyl adenosine (1000 μm) was reversible. Motility and morphology appeared to be unaffected.

AB - Trypanosoma cruzi (Brazil strain) was maintained in liver infusion tryptose medium supplemented with 5 or 10% foetal calf serum at 27 °C on a rotating shaker platform. 85 to 95% of the organisms under these conditions are epimastigotes and the medium supported logarithmic growth for up to 24 hours. The effect of S-isobutyl adenosine and SinefunginR against cultured T. cruzi epimastigotes was studied: growth rate was slowed by both in a dose-dependent fashion; 500 μm Sinefungin caused complete inhibition which was irreversible after 24-hour exposure but the effect of S-isobutyl adenosine (1000 μm) was reversible. Motility and morphology appeared to be unaffected.

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The effect of adenosine analogues on the in vitro growth of trypanosoma cruzi

Abstract

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