The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

Hua Deng; Sean Cahill; Linda Kurz; Robert Callender

doi:10.1021/ja0378714

The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

Hua Deng, Sean Cahill, Linda Kurz, Robert Callender

Biochemistry

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

Original language	English (US)
Pages (from-to)	1952-1953
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	7
DOIs	https://doi.org/10.1021/ja0378714
State	Published - Feb 25 2004

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja0378714

Cite this

@article{15fc99c6c9f84332bc4ff41aa24da8cb,

title = "The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs",

abstract = "We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.",

author = "Hua Deng and Sean Cahill and Linda Kurz and Robert Callender",

year = "2004",

month = feb,

day = "25",

doi = "10.1021/ja0378714",

language = "English (US)",

volume = "126",

pages = "1952--1953",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "7",

}

TY - JOUR

T1 - The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

AU - Deng, Hua

AU - Cahill, Sean

AU - Kurz, Linda

AU - Callender, Robert

PY - 2004/2/25

Y1 - 2004/2/25

N2 - We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

AB - We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

UR - http://www.scopus.com/inward/record.url?scp=1242297056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1242297056&partnerID=8YFLogxK

U2 - 10.1021/ja0378714

DO - 10.1021/ja0378714

M3 - Article

C2 - 14971925

AN - SCOPUS:1242297056

SN - 0002-7863

VL - 126

SP - 1952

EP - 1953

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 7

ER -

The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this