@article{64b3687ba3ec4a0aaf29b64e658c9d9e,
title = "Synthetic elaboration of native DNA by RASS (SenDr)",
abstract = "Controlled site-specific bioconjugation through chemical methods to native DNA remains an unanswered challenge. Herein, we report a simple solution to achieve this conjugation through the tactical combination of two recently developed technologies: one for the manipulation of DNA in organic media and another for the chemoselective labeling of alcohols. Reversible adsorption of solid support (RASS) is employed to immobilize DNA and facilitate its transfer into dry acetonitrile. Subsequent reaction with P(V)-based Ψ reagents takes place in high yield with exquisite selectivity for the exposed 3′ or 5′ alcohols on DNA. This two-stage process, dubbed SENDR for Synthetic Elaboration of Native DNA by RASS, can be applied to a multitude of DNA conformations and sequences with a variety of functionalized Ψ reagents to generate useful constructs.",
author = "Flood, {Dillon T.} and Knouse, {Kyle W.} and Vantourout, {Julien C.} and Seiya Kitamura and Sanchez, {Brittany B.} and Sturgell, {Emily J.} and Chen, {Jason S.} and Wolan, {Dennis W.} and Baran, {Phil S.} and Dawson, {Philip E.}",
note = "Funding Information: Financial support for this work was from NIH (GM-132787 for P.E.D, GM-118176 P.S.B and GM-136286 for D.W.W.). The authors thank Christoph Rader and Junpeng Qi for graciously providing DVD IgG. D.T.F. was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant No. UL1 TR002551 and linked award TL1 TR002551. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. All nonphotorealistic biomolecular illustrations were created with Goodsell{\textquoteright}s Illustrate. Authors are grateful to Dr. Dee-Hua Huang and Dr. Laura Pasternack (Scripps Research) for assistance with nuclear magnetic resonance (NMR) spectroscopy. Funding Information: Financial support for this work was from NIH (GM-132787 for P.E.D, GM-118176 P.S.B and GM-136286 for D.W.W.). The authors thank Christoph Rader and Junpeng Qi for graciously providing DVD IgG. D.T.F. was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant No. UL1 TR002551 and linked award TL1 TR002551. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. All nonphotorealistic biomolecular illustrations were created with Goodsell's Illustrate.77 Authors are grateful to Dr. Dee-Hua Huang and Dr. Laura Pasternack (Scripps Research) for assistance with nuclear magnetic resonance (NMR) spectroscopy. Publisher Copyright: {\textcopyright} 2020 American Chemical Society",
year = "2020",
month = oct,
day = "28",
doi = "10.1021/acscentsci.0c00680",
language = "English (US)",
volume = "6",
pages = "1789--1799",
journal = "ACS Central Science",
issn = "2374-7943",
publisher = "American Chemical Society",
number = "10",
}