Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture

R. A. Murphy; R. H. Singer; J. D. Saide; N. J. Pantazis; M. H. Blanchard; K. S. Byron; B. G. Arnason; M. Young

doi:10.1073/pnas.74.10.4496

Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture

R. A. Murphy, R. H. Singer, J. D. Saide, N. J. Pantazis, M. H. Blanchard, K. S. Byron, B. G. Arnason, M. Young

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Abstract

Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunlogically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data slso reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that if plays some role relating to the association of nerves and muscle in vivo.

Original language	English (US)
Pages (from-to)	4496-4500
Number of pages	5
Journal	Unknown Journal
Volume	74
Issue number	10
DOIs	https://doi.org/10.1073/pnas.74.10.4496
State	Published - 1977
Externally published	Yes

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title = "Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture",

abstract = "Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunlogically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data slso reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that if plays some role relating to the association of nerves and muscle in vivo.",

author = "Murphy, {R. A.} and Singer, {R. H.} and Saide, {J. D.} and Pantazis, {N. J.} and Blanchard, {M. H.} and Byron, {K. S.} and Arnason, {B. G.} and M. Young",

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T1 - Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture

AU - Murphy, R. A.

AU - Singer, R. H.

AU - Saide, J. D.

AU - Pantazis, N. J.

AU - Blanchard, M. H.

AU - Byron, K. S.

AU - Arnason, B. G.

AU - Young, M.

PY - 1977

Y1 - 1977

N2 - Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunlogically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data slso reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that if plays some role relating to the association of nerves and muscle in vivo.

AB - Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunlogically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data slso reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that if plays some role relating to the association of nerves and muscle in vivo.

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Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture

Abstract

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