TY - JOUR
T1 - Superinfection immunity of mycobacteriophage L5
T2 - applications for genetic transformation of mycobacteria
AU - Donnelly‐Wu, Mary K.
AU - Jacobs, William R.
AU - Hatfull, Graham F.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1993/2
Y1 - 1993/2
N2 - Mycobacteriophage L5 is a temperate phage of the mycobacteria that forms stable lysogens in Mycobacterium smegmatis. We show here that the 183‐amino‐acid product of L5 gene 71 confers immunity to L5 superinfection, is required for maintenance of the lysogenic state and contains a helix‐turn‐helix DNA‐binding motif—properties associated with repressors of temperate phages. We have utilized these observations to demonstrate the use of L5 gene 71 as a selectable marker for genetic transformation of the mycobacteria. Significantly, the use of L5 gene 71 as a selectable gene avoids the requirement for antibiotic‐resistance genes providing an important tool for manipulation of the pathogens Mycobacterium tuberculosis and Mycobacterium avium, and for the construction of recombinant BCG vaccines.
AB - Mycobacteriophage L5 is a temperate phage of the mycobacteria that forms stable lysogens in Mycobacterium smegmatis. We show here that the 183‐amino‐acid product of L5 gene 71 confers immunity to L5 superinfection, is required for maintenance of the lysogenic state and contains a helix‐turn‐helix DNA‐binding motif—properties associated with repressors of temperate phages. We have utilized these observations to demonstrate the use of L5 gene 71 as a selectable marker for genetic transformation of the mycobacteria. Significantly, the use of L5 gene 71 as a selectable gene avoids the requirement for antibiotic‐resistance genes providing an important tool for manipulation of the pathogens Mycobacterium tuberculosis and Mycobacterium avium, and for the construction of recombinant BCG vaccines.
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U2 - 10.1111/j.1365-2958.1993.tb01132.x
DO - 10.1111/j.1365-2958.1993.tb01132.x
M3 - Article
C2 - 8459767
AN - SCOPUS:0027536476
SN - 0950-382X
VL - 7
SP - 407
EP - 417
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 3
ER -
