Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID

Jennifer T. Aguilan; Fabian M. Dayrit; Jinhua Zhang; Milady R. Niñonuevo; Carlito B. Lebrilla

doi:10.1016/j.jasms.2005.09.009

Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID

Jennifer T. Aguilan
, Fabian M. Dayrit
, Jinhua Zhang
, Milady R. Niñonuevo
, Carlito B. Lebrilla

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.

Original language	English (US)
Pages (from-to)	96-103
Number of pages	8
Journal	Journal of the American Society for Mass Spectrometry
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/j.jasms.2005.09.009
State	Published - Jan 2006
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Spectroscopy

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@article{dd093c07623841778982c970d8bce6a5,

title = "Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID",

abstract = "Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.",

author = "Aguilan, \{Jennifer T.\} and Dayrit, \{Fabian M.\} and Jinhua Zhang and Ni{\~n}onuevo, \{Milady R.\} and Lebrilla, \{Carlito B.\}",

note = "Funding Information: This work was partially supported by the Department of Science and Technology, Philippines. The authors thank the University of California, Davis, and the Ateneo de Manila University for providing the funding for the short-term exchange visitor scholarship program for JTA. CBL thanks the NIH (USA) for its continued support. ",

year = "2006",

month = jan,

doi = "10.1016/j.jasms.2005.09.009",

language = "English (US)",

volume = "17",

pages = "96--103",

journal = "Journal of the American Society for Mass Spectrometry",

issn = "1044-0305",

publisher = "American Chemical Society",

number = "1",

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T1 - Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID

AU - Aguilan, Jennifer T.

AU - Dayrit, Fabian M.

AU - Zhang, Jinhua

AU - Niñonuevo, Milady R.

AU - Lebrilla, Carlito B.

N1 - Funding Information: This work was partially supported by the Department of Science and Technology, Philippines. The authors thank the University of California, Davis, and the Ateneo de Manila University for providing the funding for the short-term exchange visitor scholarship program for JTA. CBL thanks the NIH (USA) for its continued support.

PY - 2006/1

Y1 - 2006/1

N2 - Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.

AB - Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.

UR - https://www.scopus.com/pages/publications/29744432247

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U2 - 10.1016/j.jasms.2005.09.009

DO - 10.1016/j.jasms.2005.09.009

M3 - Article

C2 - 16352442

AN - SCOPUS:29744432247

SN - 1044-0305

VL - 17

SP - 96

EP - 103

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

IS - 1

ER -

Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID

Abstract

ASJC Scopus subject areas

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