TY - JOUR
T1 - Strategies for ultrastructural visualization of biotinated probes hybridized to messenger RNA in situ.
AU - Singer, R. H.
AU - Lawrence, J. B.
AU - Silva, F.
AU - Langevin, G. L.
AU - Pomeroy, M.
AU - Billings-Gagliardi, S.
PY - 1989
Y1 - 1989
N2 - A progressive development of the application of in situ methodology to ultrastructural procedures has resulted in the ability to detect individual molecules of mRNA with high probability. Beginning with whole-mount cells and then developing myotubes, both in culture and detergent extracted before fixation, we were able to progress to methods which allow detection of mRNA in tissue sections. Initial results confirm that the detection of mRNA in thin-sectioned tissue is very similar to observations on the extracted, cultured cells, and that the same methods of data analysis apply. Current work is devoted to the application of the methodology to other cellular structures, such as the nucleus, and to other tissue-probe systems, such as brain. Acknowledgements. The authors appreciate the skilled help from John McNeil and Shirwin Pockwinse in the laborious and time-consuming preparations of material and photography. FS was on sabbatical leave from the Department of Pathology at Southwestern Medical Center.
AB - A progressive development of the application of in situ methodology to ultrastructural procedures has resulted in the ability to detect individual molecules of mRNA with high probability. Beginning with whole-mount cells and then developing myotubes, both in culture and detergent extracted before fixation, we were able to progress to methods which allow detection of mRNA in tissue sections. Initial results confirm that the detection of mRNA in thin-sectioned tissue is very similar to observations on the extracted, cultured cells, and that the same methods of data analysis apply. Current work is devoted to the application of the methodology to other cellular structures, such as the nucleus, and to other tissue-probe systems, such as brain. Acknowledgements. The authors appreciate the skilled help from John McNeil and Shirwin Pockwinse in the laborious and time-consuming preparations of material and photography. FS was on sabbatical leave from the Department of Pathology at Southwestern Medical Center.
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U2 - 10.1007/978-3-642-74425-9_5
DO - 10.1007/978-3-642-74425-9_5
M3 - Article
C2 - 2766803
AN - SCOPUS:0024387929
SN - 0070-217X
VL - 143
SP - 55
EP - 69
JO - Current Topics in Microbiology and Immunology
JF - Current Topics in Microbiology and Immunology
ER -