Spermine potentiation of recombinant N-methyl-D-aspartate receptors is affected by subunit composition

The present study shows that both the NR1 and NR2 subunits critically affect spermine potentiation of heteromeric recombinant N-methyl-D-aspartate receptors. NR1₀₁₁, the most prominent NR1 splice variant in rat forebrain, and NR1₁₀₀, prominent in midbrain, were expressed in Xenopus oocytes singly and in combination with NR2A, NR2B, and NR2C subunits. As for NR1₀₁₁ homomers, NR1₀₁₁/NR2B receptors exhibited spermine potentiation by two mechanisms: by increasing glycine affinity and by increasing current through receptors with bound N-methyl-D-aspartate and glycine. NR1₀₁₁/NR2A receptors exhibited only the increase in glycine affinity, and NR1₀₁₁/NR2C receptors exhibited neither. As for NR1₁₀₀ homomers, NR1₁₀₀/NR2B and NR1₁₀₀/NR2A receptors exhibited spermine potentiation only by increasing the glycine affinity. Spermine produced no potentiation of NR1₁₀₀/NR2C receptors. Thus, the NR2B subunit 'permits' both forms of spermine potentiation, the NR2A subunit permits spermine potentiation only by increasing the glycine affinity, and the NR2C subunit permits neither form of potentiation. Spermine actions on NR1/NR2 showed little voltage dependence. These observations are of interest because the NR1 and NR2 subunits are differentially distributed and developmentally regulated. At early postnatal ages, NR2B subunit mRNA was more highly expressed than NR2A and NR2C mRNAs in hippocampus, neocortex, and caudate-putamen. These findings account for many of the observed differences among neurons in polyamine actions and suggest that these actions will vary in a cell-specific and age-related manner.