TY - JOUR
T1 - Sox2 gene amplification significantly impacts overall survival in serous epithelial ovarian cancer
AU - Belotte, Jimmy
AU - Fletcher, Nicole M.
AU - Alexis, Mitchell
AU - Morris, Robert T.
AU - Munkarah, Adnan R.
AU - Diamond, Michael P.
AU - Saed, Ghassan M.
N1 - Publisher Copyright:
© The Author(s) 2014.
PY - 2015/1/23
Y1 - 2015/1/23
N2 - Epithelial ovarian cancer (EOC) is the deadliest gynecologic cancer. Recently, the existence of ovarian cancer stem cells has been reported. Sox2, Nanog and Oct4 are key markers of "stemness". The objective of this study was to determine whether Sox2, Nanog, and Oct4 are associated with EOC and poor outcome. The expression of these markers was assessed by immunofluorescence staining and real-time RT-PCR in human EOC cell lines MDAH-2774 and SKOV-3, while the cancer genome atlas (TCGA) dataset was analyzed for associations with survival. Sox2, Nanog and Oct4 (POU5F1) were all detected by immunofluorescence staining and these results were confirmed by real-time RT-PCR. The TCGA dataset revealed a 26%, 9%, and 6% amplification of Sox2, Nanog and POU5F1, respectively. Additionally, K-M survival analyses showed a significant median overall survival difference (41 versus 48.3 months, P =.01) for Sox2 amplification, but not for Nanog (44.1 versus 36.2 months, P >.05) and POU5F1 (43.5 versus 45.0 months, P >.05). Our results suggest that Sox2 gene amplification significantly influences overall survival.
AB - Epithelial ovarian cancer (EOC) is the deadliest gynecologic cancer. Recently, the existence of ovarian cancer stem cells has been reported. Sox2, Nanog and Oct4 are key markers of "stemness". The objective of this study was to determine whether Sox2, Nanog, and Oct4 are associated with EOC and poor outcome. The expression of these markers was assessed by immunofluorescence staining and real-time RT-PCR in human EOC cell lines MDAH-2774 and SKOV-3, while the cancer genome atlas (TCGA) dataset was analyzed for associations with survival. Sox2, Nanog and Oct4 (POU5F1) were all detected by immunofluorescence staining and these results were confirmed by real-time RT-PCR. The TCGA dataset revealed a 26%, 9%, and 6% amplification of Sox2, Nanog and POU5F1, respectively. Additionally, K-M survival analyses showed a significant median overall survival difference (41 versus 48.3 months, P =.01) for Sox2 amplification, but not for Nanog (44.1 versus 36.2 months, P >.05) and POU5F1 (43.5 versus 45.0 months, P >.05). Our results suggest that Sox2 gene amplification significantly influences overall survival.
KW - Sox2 amplification
KW - overall survival
KW - serous epithelial ovarian cancer
UR - http://www.scopus.com/inward/record.url?scp=84919667222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919667222&partnerID=8YFLogxK
U2 - 10.1177/1933719114542021
DO - 10.1177/1933719114542021
M3 - Article
C2 - 25038052
AN - SCOPUS:84919667222
SN - 1933-7191
VL - 22
SP - 38
EP - 46
JO - Reproductive Sciences
JF - Reproductive Sciences
IS - 1
ER -
