Abstract
Lipid-mixing assay is now commonly used to study protein, temperature and ion-dependent membrane fusion events. This assay has been crucial to demonstrate the ability of neuronal and non-neuronal soluble NSF attachment receptor (SNARE) to promote spontaneous fusion of liposomes. This lipid-mixing assay is based on the fluorescence resonance energy transfer (FRET) capability between a donor fluorescent lipid and a quenching lipid. When fusion between donor fluorescent liposomes and nonfluorescent acceptor liposome occurred, FRET decreases. This assay allows a real-time reading of SNARE-mediated liposome fusion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 241-251 |
| Number of pages | 11 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 457 |
| State | Published - 2008 |
| Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Genetics