Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing

Adrian Cheng, J. Tiago Gonçalves, Peyman Golshani, Katsushi Arisaka, Carlos Portera-Cailliau

Research output: Contribution to journalArticlepeer-review

236 Scopus citations


In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalNature Methods
Issue number2
StatePublished - Feb 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing'. Together they form a unique fingerprint.

Cite this