TY - JOUR
T1 - Selection of Lectin-Resistant Mutants of Animal Cells
AU - Stanley, Pamela
N1 - Funding Information:
The writing of this chapter was supported by PHS grant CA30645 awarded by the National Cancer Institute, DHHS, and grant PCM80-23672 from the National Science Foundation. The author thanks Lisa Youkeles and Barbara Dunn for excellent technical assistance and Dr. Christine Campbell for critical comments on the manuscript. P. S. is a recipient of a senior faculty award from the American Cancer Society.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - This chapter describes a general approach, which proves to be highly successful, at isolating one family of mutants–those that express altered carbohydrate at the cell surface. Selection of a wide range of such mutants is possible by the availability of a large number of different lectins. Lectins are proteins or glycoproteins of nonimmune origin that specifically recognize certain sugar conformations. Lectin-resistant mutants are selected from a number of cell lines that exhibit very different growth properties from Chinese hamster ovary (CHO) cells—for example, mouse lymphoma cells that grow only in suspension. Cells that grow only in suspension cannot be plated in liquid medium to form colonies, and therefore, survival curves and P-tests cannot be generated by calculating relative plating efficiencies or by comparing confluency of monolayers. While cells that grow only in monolayer can more easily be pursued by the methods described for CHO cells.
AB - This chapter describes a general approach, which proves to be highly successful, at isolating one family of mutants–those that express altered carbohydrate at the cell surface. Selection of a wide range of such mutants is possible by the availability of a large number of different lectins. Lectins are proteins or glycoproteins of nonimmune origin that specifically recognize certain sugar conformations. Lectin-resistant mutants are selected from a number of cell lines that exhibit very different growth properties from Chinese hamster ovary (CHO) cells—for example, mouse lymphoma cells that grow only in suspension. Cells that grow only in suspension cannot be plated in liquid medium to form colonies, and therefore, survival curves and P-tests cannot be generated by calculating relative plating efficiencies or by comparing confluency of monolayers. While cells that grow only in monolayer can more easily be pursued by the methods described for CHO cells.
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U2 - 10.1016/S0076-6879(83)96015-9
DO - 10.1016/S0076-6879(83)96015-9
M3 - Article
C2 - 6656629
AN - SCOPUS:0021010719
SN - 0076-6879
VL - 96
SP - 157
EP - 184
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -