TY - JOUR
T1 - Sea urchin (Lytechinus pictus) late-stage histone H3 and H4 genes
T2 - Characterization and mapping of a clustered but nontandemly linked multigene family
AU - Childs, Geoffrey
AU - Nocente-Mcgrath, Catherine
AU - Lieber, Toby
AU - Holt, Curtis
AU - Knowles, James A.
N1 - Funding Information:
We thank Eric Davidson and his colleagues for providing a partial Eco RI phage library. We are particularly indebted to Ron Cohn and Larry Kedes for providing us with the L. pictus early histone gene clones used in this work as well as unpublished DNA sequences of the L. pictus early histone genes. We are grateful to Ken Krauter, Leslie Leinwand and Howard Federoff for critical review of the manuscript, John Sninsky for his help with the computer analysis of DNA sequences and Marie Lancellotti for secretarial work. This work was supported in part by grants from the National Institutes of Health and the American Cancer Society Institutional Grant. Geoffrey Childs is a recipient of a Junior Faculty Award from the American Cancer Society. Toby Lieber, Curtis Holt and Jim Knowles are supported by NIH predoctoral training grants.
PY - 1982/12
Y1 - 1982/12
N2 - We have cloned and characterized members of a small multigene family that encodes late-stage histone H3 and H4 mRNAs from the sea urchin Lytechinus pictus. Unlike their highly repetitive histone gene counterparts, which are expressed at an earlier developmental stage, late H3 and H4 histone genes are not present in tandem repeats. In addition, the late stage H3 and H4 genes are not always tightly clustered together with the H1, H2A and H2B genes as they are in early histone genes. The spacer DNA that separates adjoining H3 and H4 coding regions is not conserved between nonallelic members of the late histone gene family. We have determined the nucleotide sequence of a continuous 2100 bp segment of DNA including both H3 and H4 coding sequences, the entire spacer DNA separating the genes and surrounding nonhistone DNA. The late histone H3 and H4 genes encode proteins identical to their early gene counterparts; however, the 5′ leader sequence is shorter in late genes and the codon usage is different.
AB - We have cloned and characterized members of a small multigene family that encodes late-stage histone H3 and H4 mRNAs from the sea urchin Lytechinus pictus. Unlike their highly repetitive histone gene counterparts, which are expressed at an earlier developmental stage, late H3 and H4 histone genes are not present in tandem repeats. In addition, the late stage H3 and H4 genes are not always tightly clustered together with the H1, H2A and H2B genes as they are in early histone genes. The spacer DNA that separates adjoining H3 and H4 coding regions is not conserved between nonallelic members of the late histone gene family. We have determined the nucleotide sequence of a continuous 2100 bp segment of DNA including both H3 and H4 coding sequences, the entire spacer DNA separating the genes and surrounding nonhistone DNA. The late histone H3 and H4 genes encode proteins identical to their early gene counterparts; however, the 5′ leader sequence is shorter in late genes and the codon usage is different.
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U2 - 10.1016/0092-8674(82)90132-5
DO - 10.1016/0092-8674(82)90132-5
M3 - Article
C2 - 6297764
AN - SCOPUS:0020357952
SN - 0092-8674
VL - 31
SP - 383
EP - 393
JO - Cell
JF - Cell
IS - 2 PART 1
ER -