TY - JOUR
T1 - Screening for DNA adducts by data-dependent constant neutral loss-triple stage mass spectrometry with a linear quadrupole ion trap mass spectrometer
AU - Bessette, Erin E.
AU - Goodenough, Angela K.
AU - Langouët, Sophie
AU - Yasa, Isil
AU - Kozekov, Ivan D.
AU - Spivack, Simon D.
AU - Turesky, Robert J.
PY - 2009/1/15
Y1 - 2009/1/15
N2 - A two-dimensional linear quadrupole ion trap mass spectrometer (LIT/MS) was employed to simultaneously screen for DNA adducts of environmental, dietary, and endogenous genotoxicants, by data-dependent constant neutral loss scanning followed by triple-stage mass spectrometry (CNL-MS 3). The loss of the deoxyribose (dR) from the protonated DNA adducts ([M + H - 116] +) in the MS/MS scan mode triggered the acquisition of MS 3 production spectra of the aglycone adducts [BH 2] +. Five DNA adducts of the tobacco carcinogen 4-aminobiphenyl (4-ABP) were detected in human hepatocytes treated with 4-ABP, and three DNA adducts of the cooked-meat carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were identified in the livers of rats exposed to MeIQx, by the CNL-MS 3 scan mode. Buccal cell DNA from tobacco smokers was screened for DNA adducts of various classes of carcinogens in tobacco smoke including 4-ABP, 2-amino-9H-pyrido[2,3- b]indole (AαC), and benzo[a]pyrene (BaP); the cooked-meat carcinogens MeIQx, AαC, and 2-amino-1-methyl-6-phenylmidazo[4,5-b]pyridine (PhIP); and the lipid peroxidation products acrolein (AC) and trans-4-hydroxynonenal (HNE). The CNL-MS 3 scanning technique can be used to simultaneously screen for multiple DNA adducts derived from different classes of carcinogens, at levels of adduct modification approaching 1 adduct per 10 8 unmodified DNA bases, when 10 μg of DNA is employed for the assay.
AB - A two-dimensional linear quadrupole ion trap mass spectrometer (LIT/MS) was employed to simultaneously screen for DNA adducts of environmental, dietary, and endogenous genotoxicants, by data-dependent constant neutral loss scanning followed by triple-stage mass spectrometry (CNL-MS 3). The loss of the deoxyribose (dR) from the protonated DNA adducts ([M + H - 116] +) in the MS/MS scan mode triggered the acquisition of MS 3 production spectra of the aglycone adducts [BH 2] +. Five DNA adducts of the tobacco carcinogen 4-aminobiphenyl (4-ABP) were detected in human hepatocytes treated with 4-ABP, and three DNA adducts of the cooked-meat carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were identified in the livers of rats exposed to MeIQx, by the CNL-MS 3 scan mode. Buccal cell DNA from tobacco smokers was screened for DNA adducts of various classes of carcinogens in tobacco smoke including 4-ABP, 2-amino-9H-pyrido[2,3- b]indole (AαC), and benzo[a]pyrene (BaP); the cooked-meat carcinogens MeIQx, AαC, and 2-amino-1-methyl-6-phenylmidazo[4,5-b]pyridine (PhIP); and the lipid peroxidation products acrolein (AC) and trans-4-hydroxynonenal (HNE). The CNL-MS 3 scanning technique can be used to simultaneously screen for multiple DNA adducts derived from different classes of carcinogens, at levels of adduct modification approaching 1 adduct per 10 8 unmodified DNA bases, when 10 μg of DNA is employed for the assay.
UR - http://www.scopus.com/inward/record.url?scp=60549090590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60549090590&partnerID=8YFLogxK
U2 - 10.1021/ac802096p
DO - 10.1021/ac802096p
M3 - Article
C2 - 19086795
AN - SCOPUS:60549090590
SN - 0003-2700
VL - 81
SP - 809
EP - 819
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 2
ER -