TY - JOUR
T1 - Role of ligandin in transfer of bilirubin from plasma into liver
AU - Wolkoff, A. W.
AU - Goresky, C. A.
AU - Sellin, J.
PY - 1979
Y1 - 1979
N2 - Multiple-indicator dilution studies of labeled bilirubin uptake were carried out on isolated perfused rat livers with variable ligandin concentrations (from normal and thyroidectomized animals with and without phenobarbital pretreatment). Ligandin concentrations, measured immunologically, increased 25% after thyroidectomy and approximately doubled after phenobarbital pretreatment but decreased to normal during perfusion in the thyroidectomized nonpretreated group. A distributed two-compartment model was fitted to the dilution data and estimates of influx, efflux, and sequestration coefficients were obtained. Influx and sequestration coefficients did not vary significantly between the groups. Efflux coefficients were significantly smaller (P<0.001), and hepatic ligandin concentrations were significantly larger (P<0.001) in phenobarbital-treated rats than in other groups. The efflux coefficient varied inversely with ligandin concentration and the volume of distribution in tissue, as perceived from the plasma space, increased in proportion to the concentration of ligandin. The increased net uptake of tracer bilirubin by the liver of phenobarbital-pretreated animals is due to decreased tracer efflux secondary to the increase in intracellular binding of bilirubin by ligandin.
AB - Multiple-indicator dilution studies of labeled bilirubin uptake were carried out on isolated perfused rat livers with variable ligandin concentrations (from normal and thyroidectomized animals with and without phenobarbital pretreatment). Ligandin concentrations, measured immunologically, increased 25% after thyroidectomy and approximately doubled after phenobarbital pretreatment but decreased to normal during perfusion in the thyroidectomized nonpretreated group. A distributed two-compartment model was fitted to the dilution data and estimates of influx, efflux, and sequestration coefficients were obtained. Influx and sequestration coefficients did not vary significantly between the groups. Efflux coefficients were significantly smaller (P<0.001), and hepatic ligandin concentrations were significantly larger (P<0.001) in phenobarbital-treated rats than in other groups. The efflux coefficient varied inversely with ligandin concentration and the volume of distribution in tissue, as perceived from the plasma space, increased in proportion to the concentration of ligandin. The increased net uptake of tracer bilirubin by the liver of phenobarbital-pretreated animals is due to decreased tracer efflux secondary to the increase in intracellular binding of bilirubin by ligandin.
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U2 - 10.1152/ajpendo.1979.236.6.e638
DO - 10.1152/ajpendo.1979.236.6.e638
M3 - Article
C2 - 375751
AN - SCOPUS:0018485896
SN - 0363-6100
VL - 5
SP - E638-E648
JO - American Journal of Physiology Endocrinology Metabolism and Gastrointestinal Physiology
JF - American Journal of Physiology Endocrinology Metabolism and Gastrointestinal Physiology
IS - 6
ER -