Requirement of A1-α for bacillus Calmette-Guérin-mediated protection of macrophages against nitric oxide-induced apoptosis

S. Kausalya, R. Somogyi, A. Orlofsky, M. B. Prystowsky

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The role of apoptosis in regulating the course of intracellular microbial infection is not well understood. We studied the relationship between apoptotic regulation and bacillus Calmette-Guérin (BCG) treatment in murine peritoneal exudate macrophages (PEM) and the J774 macrophage cell line. In both PEM and J774 cells, mRNA expression of the anti-apoptotic gene, A1, was selectively induced by BCG treatment as compared with other bcl2 family members (bcl-w, bcl-2, bcl-xl, bcl-xs, bax, bak, bad). In PEM, A1 expression was maximal by 8 h postinfection and was abrogated by the proteasomal inhibitor MG-132. The induction was independent of protein synthesis as well as the p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase pathways and did not require live organism. Three genes encoding closely related isoforms of A1 were all expressed; however, the A1-a isoform displayed the greatest fold induction in PEM. BCG-induced A1 expression was associated with protection of host macrophages from NO-mediated apoptosis in both PEM and J774 cells. BCG-mediated protection was abrogated in PEM derived from A1-a-/- mice, indicating a requirement of A1-a for survival of inflammatory macrophages.

Original languageEnglish (US)
Pages (from-to)4721-4727
Number of pages7
JournalJournal of Immunology
Volume166
Issue number7
DOIs
StatePublished - Apr 1 2001

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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