Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes

Achilles A. Demetriou; James F. Whiting; David Feldman; Stanley M. Levenson; Namita Roy Chowdhury; Albert D. Moscioni; Michael Kram; Jayanta Roy Chowdhury

doi:10.1126/science.2426782

Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes

Achilles A. Demetriou
, James F. Whiting
, David Feldman
, Stanley M. Levenson
, Namita Roy Chowdhury
, Albert D. Moscioni
, Michael Kram
, Jayanta Roy Chowdhury

Medicine

Research output: Contribution to journal › Article › peer-review

275 Scopus citations

Abstract

Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

Original language	English (US)
Pages (from-to)	1190-1192
Number of pages	3
Journal	Science
Volume	233
Issue number	4769
DOIs	https://doi.org/10.1126/science.2426782
State	Published - 1986

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title = "Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes",

abstract = "Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.",

author = "Demetriou, \{Achilles A.\} and Whiting, \{James F.\} and David Feldman and Levenson, \{Stanley M.\} and Chowdhury, \{Namita Roy\} and Moscioni, \{Albert D.\} and Michael Kram and Chowdhury, \{Jayanta Roy\}",

year = "1986",

doi = "10.1126/science.2426782",

language = "English (US)",

volume = "233",

pages = "1190--1192",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

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T1 - Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes

AU - Demetriou, Achilles A.

AU - Whiting, James F.

AU - Feldman, David

AU - Levenson, Stanley M.

AU - Chowdhury, Namita Roy

AU - Moscioni, Albert D.

AU - Kram, Michael

AU - Chowdhury, Jayanta Roy

PY - 1986

Y1 - 1986

N2 - Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

AB - Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

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UR - https://www.scopus.com/pages/publications/0022460518#tab=citedBy

U2 - 10.1126/science.2426782

DO - 10.1126/science.2426782

M3 - Article

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AN - SCOPUS:0022460518

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VL - 233

SP - 1190

EP - 1192

JO - Science

JF - Science

IS - 4769

ER -

Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes

Abstract

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