TY - JOUR
T1 - Reference lineshape adjusted difference NMR spectroscopy. II. experimental verification
AU - Wouters, Jan M.
AU - Petersson, G. A.
AU - Agosta, William C.
AU - Field, F. H.
AU - Gibbons, W. A.
AU - Wyssbrod, Herman
AU - Cowburn, David
N1 - Funding Information:
ACKNOWLEDGMENTS We gratefully acknowledge financial support from the Uniroyal Corporation and from Wesleyan University. The lOO-MHz NMR spectrometer at Wesleyan University, which was obtained through the NSF under Grant MP75-06524 was used to test some of the ideas developed in this paper. Research at The Rockefeller University was supported by the NIH under Grant AM 2493. The 220-MHz NMR facilities located at The Rockefeller University and operated by a consortium are supported by the NSF under Grant BMS74-12247.
PY - 1977/10
Y1 - 1977/10
N2 - The accuracy and reliability of difference NMR spectroscopy can be substantially improved by a simple correction based on changes in the lineshape of an internal reference line. This reference lineshape adjusted (RLSA) method is experimentally demonstrated to behave according to theoretical predictions under variations in drift of the main magnetic field, in rf power, in homogeneity of the main field, in spinning side bands, in detector phase, and in sweep rate. The RLSA method is experimentally demonstrated to automatically compensate for changes in all these instrumental parameters provided H, is sufficiently small that saturation is avoided. In addition, the internal reference must be constant in composition, concentration, and resonance position, and must be totally resolved from the rest of the spectrum. Under these conditions, the RLSA method is shown to give difference spectra that are visibly superior to simple difference spectra.
AB - The accuracy and reliability of difference NMR spectroscopy can be substantially improved by a simple correction based on changes in the lineshape of an internal reference line. This reference lineshape adjusted (RLSA) method is experimentally demonstrated to behave according to theoretical predictions under variations in drift of the main magnetic field, in rf power, in homogeneity of the main field, in spinning side bands, in detector phase, and in sweep rate. The RLSA method is experimentally demonstrated to automatically compensate for changes in all these instrumental parameters provided H, is sufficiently small that saturation is avoided. In addition, the internal reference must be constant in composition, concentration, and resonance position, and must be totally resolved from the rest of the spectrum. Under these conditions, the RLSA method is shown to give difference spectra that are visibly superior to simple difference spectra.
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U2 - 10.1016/0022-2364(77)90259-1
DO - 10.1016/0022-2364(77)90259-1
M3 - Article
AN - SCOPUS:0043283987
SN - 0022-2364
VL - 28
SP - 93
EP - 104
JO - Journal of Magnetic Resonance (1969)
JF - Journal of Magnetic Resonance (1969)
IS - 1
ER -