Quantitative mRNA imaging throughout the entire Drosophila brain

Xi Long; Jennifer Colonell; Allan M. Wong; Robert H. Singer; Timothée Lionnet

doi:10.1038/nmeth.4309

Quantitative mRNA imaging throughout the entire Drosophila brain

Xi Long, Jennifer Colonell, Allan M. Wong, Robert H. Singer, Timothée Lionnet

Cell Biology

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).

Original language	English (US)
Pages (from-to)	703-706
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.4309
State	Published - Jun 29 2017

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.4309

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AU - Colonell, Jennifer

AU - Wong, Allan M.

AU - Singer, Robert H.

AU - Lionnet, Timothée

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Quantitative mRNA imaging throughout the entire Drosophila brain

Abstract

ASJC Scopus subject areas

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