@article{8721f64c4a8d446b9f4157caadd852bf,
title = "Quantitative mRNA imaging throughout the entire Drosophila brain",
abstract = "We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).",
author = "Xi Long and Jennifer Colonell and Wong, {Allan M.} and Singer, {Robert H.} and Timoth{\'e}e Lionnet",
note = "Funding Information: We thank members of the former Transcription Imaging Consortium, M. Rosbash, M. Diaz, K. Abruzzi, L. Lavis, T. Brown, U. Heberlein, T. Harris and Y. Wu, for valuable suggestions. We are grateful to A. Nern for providing the Mi4, Mi1 and Mi9 transgenic flies and to G. Henry and F. Davis for providing transcriptome profiling information. We thank K. Close for assistance with Drosophila dissections and A. Lemire, L. Wang, R. Ray, K. Aswath and X. Zhang for assistance with molecular biology and characterizing transgenic flies. We also thank E. Betzig J. Jordan, and D. Milkie for consultation on the design of the BB-SIM microscope and the control software for the system; L. Shao for structured illumination analysis code; and A. Taylor for assistance with confocal imaging. Funding for this work was provided by the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2017 Nature America, Inc., part of Springer Nature. All rights reserved.",
year = "2017",
month = jun,
day = "29",
doi = "10.1038/nmeth.4309",
language = "English (US)",
volume = "14",
pages = "703--706",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "7",
}