TY - JOUR
T1 - Purification and reconstitution of chloride channels from kidney and trachea
AU - Landry, Donald W.
AU - Akabas, Myles H.
AU - Redhead, Christopher
AU - Edelman, Aleksander
AU - Cragoe, Edward J.
AU - Al-Awqati, Qais
PY - 1989
Y1 - 1989
N2 - Chloride channels mediate absorption and secretion of fluid in epithelia, and the regulation of these channels is now known to be defective in cystic fibrosis. Indanyloxyacetic acid 94 (IAA-94) is a high-affinity ligand for the chloride channel, and an affinity resin based on that structure was developed. Solubilized proteins from kidney and trachea membranes were applied to the affinity matrix, and four proteins with apparent molecular masses of 97, 64, 40, and 27 kilodaltons were eluted from the column by excess IAA-94. A potential-dependent 36Cl- uptake was observed after reconstituting these proteins into liposomes. Three types of chloride channels with single-channel conductances of 26, 100, and 400 picosiemens were observed after fusion of these liposomes with planar lipid bilayers. Similar types of chloride channels have been observed in epithelia.
AB - Chloride channels mediate absorption and secretion of fluid in epithelia, and the regulation of these channels is now known to be defective in cystic fibrosis. Indanyloxyacetic acid 94 (IAA-94) is a high-affinity ligand for the chloride channel, and an affinity resin based on that structure was developed. Solubilized proteins from kidney and trachea membranes were applied to the affinity matrix, and four proteins with apparent molecular masses of 97, 64, 40, and 27 kilodaltons were eluted from the column by excess IAA-94. A potential-dependent 36Cl- uptake was observed after reconstituting these proteins into liposomes. Three types of chloride channels with single-channel conductances of 26, 100, and 400 picosiemens were observed after fusion of these liposomes with planar lipid bilayers. Similar types of chloride channels have been observed in epithelia.
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U2 - 10.1126/science.2472007
DO - 10.1126/science.2472007
M3 - Article
C2 - 2472007
AN - SCOPUS:0024393477
SN - 0036-8075
VL - 244
SP - 1469
EP - 1472
JO - Science
JF - Science
IS - 4911
ER -