Pulmonary cryptococcosis induces chitinase in the rat

Alfin G. Vicencio, Swati Narain, Zhongfang Du, Wang Y. Zeng, James Ritch, Arturo Casadevall, David L. Goldman

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Background: We previously demonstrated that chronic pulmonary infection with Cryptococcus neoformans results in enhanced allergic inflammation and airway hyperreactivity in a rat model. Because the cell wall of C. neoformans consists of chitin, and since acidic mammalian chitinase (AMCase) has recently been implicated as a novel mediator of asthma, we sought to determine whether such infection induces chitinase activity and expression of AMCase in the rat.Methods: We utilized a previously-established model of chronic C. neoformans pulmonary infection in the rat to analyze the activity, expression and localization of AMCase.Results: Our studies indicate that intratracheal inoculation of C. neoformans induces chitinase activity within the lung and bronchoalveolar lavage fluid of infected rats. Chitinase activity is also elicited by pulmonary infection with other fungi (e.g. C. albicans), but not by the inoculation of dead organisms. Enhanced chitinase activity reflects increased AMCase expression by airway epithelial cells and alveolar macrophages. Systemic cryptococcosis is not associated with increased pulmonary chitinase activity or AMCase expression.Conclusion: Our findings indicate a possible link between respiratory fungal infections, including C. neoformans, and asthma through the induction of AMCase.

Original languageEnglish (US)
Article number40
JournalRespiratory Research
StatePublished - May 15 2008

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine


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