Protein production and purification

Susanne Gräslund; Pär Nordlund; Johan Weigelt; B. Martin Hallberg; James Bray; Opher Gileadi; Stefan Knapp; Udo Oppermann; Cheryl Arrowsmith; Raymond Hui; Jinrong Ming; Sirano dhe-Paganon; Hee Won Park; Alexei Savchenko; Adelinda Yee; Aled Edwards; Renaud Vincentelli; Christian Cambillau; Rosalind Kim; Sung Hou Kim; Zihe Rao; Yunyu Shi; Thomas C. Terwilliger; Chang Yub Kim; Li Wei Hung; Geoffrey S. Waldo; Yoav Peleg; Shira Albeck; Tamar Unger; Orly Dym; Jaime Prilusky; Joel L. Sussman; Ray C. Stevens; Scott A. Lesley; Ian A. Wilson; Andrzej Joachimiak; Frank Collart; Irina Dementieva; Mark I. Donnelly; William H. Eschenfeldt; Youngchang Kim; Lucy Stols; Ruying Wu; Min Zhou; Stephen K. Burley; J. Spencer Emtage; J. Michael Sauder; Devon Thompson; Kevin Bain; John Luz; Tarun Gheyi; Fred Zhang; Shane Atwell; Steven C. Almo; Jeffrey B. Bonanno; Andras Fiser; Sivasubramanian Swaminathan; F. William Studier; Mark R. Chance; Andrej Sali; Thomas B. Acton; Rong Xiao; Li Zhao; Li Chung Ma; John F. Hunt; Liang Tong; Kellie Cunningham; Masayori Inouye; Stephen Anderson; Heleema Janjua; Ritu Shastry; Chi Kent Ho; Dongyan Wang; Huang Wang; Mei Jiang; Gaetano T. Montelione; David I. Stuart; Raymond J. Owens; Susan Daenke; Anja Schütz; Udo Heinemann; Shigeyuki Yokoyama; Konrad Büssow; Kristin C. Gunsalus

doi:10.1038/nmeth.f.202

Protein production and purification

Susanne Gräslund, Pär Nordlund, Johan Weigelt, B. Martin Hallberg, James Bray, Opher Gileadi, Stefan Knapp, Udo Oppermann, Cheryl Arrowsmith, Raymond Hui, Jinrong Ming, Sirano dhe-Paganon, Hee Won Park, Alexei Savchenko, Adelinda Yee, Aled Edwards, Renaud Vincentelli, Christian Cambillau, Rosalind Kim, Sung Hou KimZihe Rao, Yunyu Shi, Thomas C. Terwilliger, Chang Yub Kim, Li Wei Hung, Geoffrey S. Waldo, Yoav Peleg, Shira Albeck, Tamar Unger, Orly Dym, Jaime Prilusky, Joel L. Sussman, Ray C. Stevens, Scott A. Lesley, Ian A. Wilson, Andrzej Joachimiak, Frank Collart, Irina Dementieva, Mark I. Donnelly, William H. Eschenfeldt, Youngchang Kim, Lucy Stols, Ruying Wu, Min Zhou, Stephen K. Burley, J. Spencer Emtage, J. Michael Sauder, Devon Thompson, Kevin Bain, John Luz, Tarun Gheyi, Fred Zhang, Shane Atwell, Steven C. Almo, Jeffrey B. Bonanno, Andras Fiser, Sivasubramanian Swaminathan, F. William Studier, Mark R. Chance, Andrej Sali, Thomas B. Acton, Rong Xiao, Li Zhao, Li Chung Ma, John F. Hunt, Liang Tong, Kellie Cunningham, Masayori Inouye, Stephen Anderson, Heleema Janjua, Ritu Shastry, Chi Kent Ho, Dongyan Wang, Huang Wang, Mei Jiang, Gaetano T. Montelione, David I. Stuart, Raymond J. Owens, Susan Daenke, Anja Schütz, Udo Heinemann, Shigeyuki Yokoyama, Konrad Büssow, Kristin C. Gunsalus

Research output: Contribution to journal › Review article › peer-review

698 Scopus citations

Abstract

In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

Original language	English (US)
Pages (from-to)	135-146
Number of pages	12
Journal	Nature Methods
Volume	5
Issue number	2
DOIs	https://doi.org/10.1038/nmeth.f.202
State	Published - Feb 2008

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.f.202

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Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M., Bray, J., Gileadi, O., Knapp, S., Oppermann, U., Arrowsmith, C., Hui, R., Ming, J., dhe-Paganon, S., Park, H. W., Savchenko, A., Yee, A., Edwards, A., Vincentelli, R., Cambillau, C., Kim, R., ... Gunsalus, K. C. (2008). Protein production and purification. Nature Methods, 5(2), 135-146. https://doi.org/10.1038/nmeth.f.202

Gräslund, S, Nordlund, P, Weigelt, J, Hallberg, BM, Bray, J, Gileadi, O, Knapp, S, Oppermann, U, Arrowsmith, C, Hui, R, Ming, J, dhe-Paganon, S, Park, HW, Savchenko, A, Yee, A, Edwards, A, Vincentelli, R, Cambillau, C, Kim, R, Kim, SH, Rao, Z, Shi, Y, Terwilliger, TC, Kim, CY, Hung, LW, Waldo, GS, Peleg, Y, Albeck, S, Unger, T, Dym, O, Prilusky, J, Sussman, JL, Stevens, RC, Lesley, SA, Wilson, IA, Joachimiak, A, Collart, F, Dementieva, I, Donnelly, MI, Eschenfeldt, WH, Kim, Y, Stols, L, Wu, R, Zhou, M, Burley, SK, Emtage, JS, Sauder, JM, Thompson, D, Bain, K, Luz, J, Gheyi, T, Zhang, F, Atwell, S, Almo, SC , Bonanno, JB , Fiser, A, Swaminathan, S, Studier, FW, Chance, MR, Sali, A, Acton, TB, Xiao, R, Zhao, L, Ma, LC, Hunt, JF, Tong, L, Cunningham, K, Inouye, M, Anderson, S, Janjua, H, Shastry, R, Ho, CK, Wang, D, Wang, H, Jiang, M, Montelione, GT, Stuart, DI, Owens, RJ, Daenke, S, Schütz, A, Heinemann, U, Yokoyama, S, Büssow, K & Gunsalus, KC 2008, 'Protein production and purification', Nature Methods, vol. 5, no. 2, pp. 135-146. https://doi.org/10.1038/nmeth.f.202

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abstract = "In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.",

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AU - Weigelt, Johan

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AU - Gileadi, Opher

AU - Knapp, Stefan

AU - Oppermann, Udo

AU - Arrowsmith, Cheryl

AU - Hui, Raymond

AU - Ming, Jinrong

AU - dhe-Paganon, Sirano

AU - Park, Hee Won

AU - Savchenko, Alexei

AU - Yee, Adelinda

AU - Edwards, Aled

AU - Vincentelli, Renaud

AU - Cambillau, Christian

AU - Kim, Rosalind

AU - Kim, Sung Hou

AU - Rao, Zihe

AU - Shi, Yunyu

AU - Terwilliger, Thomas C.

AU - Kim, Chang Yub

AU - Hung, Li Wei

AU - Waldo, Geoffrey S.

AU - Peleg, Yoav

AU - Albeck, Shira

AU - Unger, Tamar

AU - Dym, Orly

AU - Prilusky, Jaime

AU - Sussman, Joel L.

AU - Stevens, Ray C.

AU - Lesley, Scott A.

AU - Wilson, Ian A.

AU - Joachimiak, Andrzej

AU - Collart, Frank

AU - Dementieva, Irina

AU - Donnelly, Mark I.

AU - Eschenfeldt, William H.

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AU - Xiao, Rong

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AU - Montelione, Gaetano T.

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AU - Owens, Raymond J.

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AU - Schütz, Anja

AU - Heinemann, Udo

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AU - Büssow, Konrad

AU - Gunsalus, Kristin C.

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Protein production and purification

Abstract

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