Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells

Chan Jung Chang, Koyel Mitra, Mariko Koya, Michelle Velho, Romain Desprat, Jack Lenz, Eric E. Bouhassira

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


We have previously shown that human embryonic stem cells can be differentiated into embryonic and fetal type of red blood cells that sequentially express three types of hemoglobins recapitulating early human erythropoiesis. We report here that we have produced iPS from three somatic cell types: adult skin fibroblasts as well as embryonic and fetal mesenchymal stem cells. We show that regardless of the age of the donor cells, the iPS produced are fully reprogrammed into a pluripotent state that is undistinguishable from that of hESCs by low and high-throughput expression and detailed analysis of globin expression patterns by HPLC. This suggests that reprogramming with the four original Yamanaka pluripotency factors leads to complete erasure of all functionally important epigenetic marks associated with erythroid differentiation regardless of the age or the tissue type of the donor cells, at least as detected in these assays. The ability to produce large number of erythroid cells with embryonic and fetal-like characteristics is likely to have many translational applications.

Original languageEnglish (US)
Article numbere25761
JournalPloS one
Issue number10
StatePublished - Oct 13 2011

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General


Dive into the research topics of 'Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells'. Together they form a unique fingerprint.

Cite this