Preparation and characterization of 15N2H3H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2

Walter Bodmer; Carl Schildkraut

doi:10.1016/0003-2697(64)90051-X

Preparation and characterization of ¹⁵N²H³H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2

Walter Bodmer
, Carl Schildkraut

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Bacillus subtilis and T2 phage have been grown on a medium containing the following isotopically labeled compounds: ¹⁵N²H₄Cl, thymidine-³H, deuterated sugars, and deuterated amino acids. The growth rates, titers, and thymidine-³H uptake are reported. ¹⁵N²H³H-DNA has been isolated from these two organisms. Some physical properties of these DNA samples are reported. Studies on genetic activity of the B. subtilis DNA do not reveal any change produced by the substitution of ¹⁵N²H³H for ¹⁴N¹H.

Original language	English (US)
Pages (from-to)	229-243
Number of pages	15
Journal	Analytical Biochemistry
Volume	8
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(64)90051-X
State	Published - Jun 1964
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(64)90051-X

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title = "Preparation and characterization of 15N2H3H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2",

abstract = "Bacillus subtilis and T2 phage have been grown on a medium containing the following isotopically labeled compounds: 15N2H4Cl, thymidine-3H, deuterated sugars, and deuterated amino acids. The growth rates, titers, and thymidine-3H uptake are reported. 15N2H3H-DNA has been isolated from these two organisms. Some physical properties of these DNA samples are reported. Studies on genetic activity of the B. subtilis DNA do not reveal any change produced by the substitution of 15N2H3H for 14N1H.",

author = "Walter Bodmer and Carl Schildkraut",

note = "Funding Information: We are grateful to Drs. C. C. Richardson, A. T. Ganesan, J. Lederberg, and A. Kornberg for their helpful suggestions. These studies were supported by grants to the department of Genetics (NIH Training Grant 2G 295, and Research Grant C4496; and NSF Grant G-64111, and to the Department of Biochemistry (NIH Grant GM 07581).",

year = "1964",

month = jun,

doi = "10.1016/0003-2697(64)90051-X",

language = "English (US)",

volume = "8",

pages = "229--243",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Preparation and characterization of 15N2H3H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2

AU - Bodmer, Walter

AU - Schildkraut, Carl

N1 - Funding Information: We are grateful to Drs. C. C. Richardson, A. T. Ganesan, J. Lederberg, and A. Kornberg for their helpful suggestions. These studies were supported by grants to the department of Genetics (NIH Training Grant 2G 295, and Research Grant C4496; and NSF Grant G-64111, and to the Department of Biochemistry (NIH Grant GM 07581).

PY - 1964/6

Y1 - 1964/6

N2 - Bacillus subtilis and T2 phage have been grown on a medium containing the following isotopically labeled compounds: 15N2H4Cl, thymidine-3H, deuterated sugars, and deuterated amino acids. The growth rates, titers, and thymidine-3H uptake are reported. 15N2H3H-DNA has been isolated from these two organisms. Some physical properties of these DNA samples are reported. Studies on genetic activity of the B. subtilis DNA do not reveal any change produced by the substitution of 15N2H3H for 14N1H.

AB - Bacillus subtilis and T2 phage have been grown on a medium containing the following isotopically labeled compounds: 15N2H4Cl, thymidine-3H, deuterated sugars, and deuterated amino acids. The growth rates, titers, and thymidine-3H uptake are reported. 15N2H3H-DNA has been isolated from these two organisms. Some physical properties of these DNA samples are reported. Studies on genetic activity of the B. subtilis DNA do not reveal any change produced by the substitution of 15N2H3H for 14N1H.

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U2 - 10.1016/0003-2697(64)90051-X

DO - 10.1016/0003-2697(64)90051-X

M3 - Article

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AN - SCOPUS:50549206306

SN - 0003-2697

VL - 8

SP - 229

EP - 243

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Preparation and characterization of ¹⁵N²H³H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2

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