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Performance benchmarking of four cell-free protein expression systems
Dejan Gagoski
, Mark E. Polinkovsky
, Sergey Mureev
, Anne Kunert
, Wayne Johnston
, Yann Gambin
, Kirill Alexandrov
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Contribution to journal
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Article
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peer-review
59
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Keyphrases
Escherichia Coli
100%
HeLa
100%
Cell-free Protein Synthesis
100%
Performance Benchmarking
100%
Wheat Germ
75%
Aggregation Propensity
75%
High-level Expression
50%
Expression Yield
50%
Eukaryotic Proteins
25%
Expression Level
25%
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
25%
Paucity
25%
Western Blot Analysis
25%
Cytosolic Proteins
25%
Systems-based
25%
Structural Biology
25%
Eukaryotic Expression System
25%
Translation Initiation
25%
Cell-free System
25%
Multi-domain
25%
Prokaryotic Organisms
25%
Eukaryotic Organisms
25%
Leishmania
25%
Monodisperse
25%
Protein Yield
25%
Single-molecule Fluorescence Imaging
25%
Initiation Sequence
25%
Directed Protein Evolution
25%
High Integrity
25%
GFP Tag
25%
Protein Products
25%
Translation Product
25%
Agricultural and Biological Sciences
Escherichia coli
100%
HeLa
100%
Cell-Free Protein Synthesis
100%
Wheat Germ
75%
Biological Sciences
25%
Copurification
25%
Protein Product
25%
Fluorescence Spectroscopy
25%
Western Blotting
25%
Cell-Free System
25%
Leishmania
25%
Biochemistry, Genetics and Molecular Biology
Escherichia coli
100%
Gene Expression System
100%
Cell-Free Protein Synthesis
100%
Copurification
25%
Polyacrylamide Gel Electrophoresis
25%
Western Blot
25%
Translation Initiation
25%
Leishmania
25%
Cell-Free System
25%
Fluorescence Spectroscopy
25%
Expression Level
25%