PD-L1 is an activation-independent marker of brown adipocytes

Jessica R. Ingram; Michael Dougan; Mohammad Rashidian; Marko Knoll; Edmund J. Keliher; Sarah Garrett; Scott Garforth; Olga S. Blomberg; Camilo Espinosa; Atul Bhan; Steven C. Almo; Ralph Weissleder; Harvey Lodish; Stephanie K. Dougan; Hidde L. Ploegh

doi:10.1038/s41467-017-00799-8

PD-L1 is an activation-independent marker of brown adipocytes

Jessica R. Ingram, Michael Dougan, Mohammad Rashidian, Marko Knoll, Edmund J. Keliher, Sarah Garrett, Scott Garforth, Olga S. Blomberg, Camilo Espinosa, Atul Bhan, Steven C. Almo, Ralph Weissleder, Harvey Lodish, Stephanie K. Dougan, Hidde L. Ploegh

Biochemistry

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.

Original language	English (US)
Article number	647
Journal	Nature communications
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41467-017-00799-8
State	Published - Dec 1 2017

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41467-017-00799-8

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title = "PD-L1 is an activation-independent marker of brown adipocytes",

abstract = "Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.",

author = "Ingram, {Jessica R.} and Michael Dougan and Mohammad Rashidian and Marko Knoll and Keliher, {Edmund J.} and Sarah Garrett and Scott Garforth and Blomberg, {Olga S.} and Camilo Espinosa and Atul Bhan and Almo, {Steven C.} and Ralph Weissleder and Harvey Lodish and Dougan, {Stephanie K.} and Ploegh, {Hidde L.}",

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doi = "10.1038/s41467-017-00799-8",

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AU - Ingram, Jessica R.

AU - Dougan, Michael

AU - Rashidian, Mohammad

AU - Knoll, Marko

AU - Keliher, Edmund J.

AU - Garrett, Sarah

AU - Garforth, Scott

AU - Blomberg, Olga S.

AU - Espinosa, Camilo

AU - Bhan, Atul

AU - Almo, Steven C.

AU - Weissleder, Ralph

AU - Lodish, Harvey

AU - Dougan, Stephanie K.

AU - Ploegh, Hidde L.

PY - 2017/12/1

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N2 - Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.

AB - Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.

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PD-L1 is an activation-independent marker of brown adipocytes

Abstract

ASJC Scopus subject areas

UN SDGs

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