@article{e847e2168b3d482392390ad7729d2c90,
title = "PD-L1 is an activation-independent marker of brown adipocytes",
abstract = "Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.",
author = "Ingram, {Jessica R.} and Michael Dougan and Mohammad Rashidian and Marko Knoll and Keliher, {Edmund J.} and Sarah Garrett and Scott Garforth and Blomberg, {Olga S.} and Camilo Espinosa and Atul Bhan and Almo, {Steven C.} and Ralph Weissleder and Harvey Lodish and Dougan, {Stephanie K.} and Ploegh, {Hidde L.}",
note = "Funding Information: We thank Charles Shoemaker, Jean Mukherjee and Jacqueline Tremblay at Tufts Cummings Veterinary School for their assistance with alpaca immunizations. We thank Peter Sage and Arlene Sharpe (Harvard Medical School, Boston, MA) for providing PDL1 knockout mice, as well as Camille Le Gall, Alica Linnebacher, Christina Martone, Nora Kory, Patti Wisniewski and the Whitehead Flow Cytometry Core (Whitehead Institute for Biomedical Research, Cambridge, MA), Girijesh Buruzula at the Joslin Diabetes Center Flow Cytometry Core (NIH Shared Instrument grant award number 1S100D021740-01), Wendy Salmon and the WM Keck Microscopy Facility (Whitehead Institute) and Howard Mak and Scott Malstrom of the Koch Institute Animal Imaging and Preclinical Testing (MIT) for technical assistance. Funding was provided by the Lustgarten Foundation (H.L.P), an NIH Pioneer award (H.L.P.), the AACR-Pancreatic Cancer Action Network (S.K.D), the Ludwig Cancer Research Postdoctoral Fellowship (J. R.I), NIH training grant 1F32CA210568-01 (M.D.), the American Gastroenterology Association Research Scholars Award (M.D.), NIH/NIDDK 2R01 DK047618-28 (H.L.), the Deutsche Forschungsgemeinschaft (D.F.G) fellowship Kn1106/1-1 (M.K.), NIH HG008325, GM094662 and GM094665 (S.C.A.) and the CRI Postdoctoral Fellowship (M.R.); we also acknowledge support from the Albert Einstein Cancer Center (P30CA013330). Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41467-017-00799-8",
language = "English (US)",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}
